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Literature summary for 3.4.11.25 extracted from

  • Geueke, B.; Namoto, K.; Seebach, D.; Kohler, H.P.
    A novel beta-peptidyl aminopeptidase (BapA) from strain 3-2W4 cleaves peptide bonds of synthetic beta-tri- and beta-dipeptides (2005), J. Bacteriol., 187, 5910-5917.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.25

Activating Compound

Activating Compound Comment Organism Structure
additional information no activating effects are observed when the enzyme assay is performed in the presence of 1 mM of EDTA, beta-mercaptoethanol, 1,4-dithio-DL-threitol, or phenylmethylsulfonyl fluoride Sphingosinicella xenopeptidilytica

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibitory effects are observed when the enzyme assay is performed in the presence of 1 mM of EDTA, CaCl2, MgCl2, ZnCl2, CoCl2, FeCl2, MnCl2, CuCl2, beta-mercaptoethanol, 1,4-dithio-DL-threitol, or phenylmethylsulfonyl fluoride Sphingosinicella xenopeptidilytica

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no activating effects are observed when the enzyme assay is performed in the presence of 1 mM of CaCl2, MgCl2, ZnCl2, CoCl2, FeCl2, MnCl2 or CuCl2 Sphingosinicella xenopeptidilytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
 x * 12000 + x * 27000, SDS-PAGE Sphingosinicella xenopeptidilytica
27000
-
 x * 12000 + x * 27000, SDS-PAGE Sphingosinicella xenopeptidilytica

Organism

Organism UniProt Comment Textmining
Sphingosinicella xenopeptidilytica Q52VH2
-
-
Sphingosinicella xenopeptidilytica 3-2W4 Q52VH2
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the gene encodes a beta-peptidyl aminopeptidase (BapA) of 402 amino acids that is synthesized as preprotein with a signal sequence of 29 amino acids. The enzyme is cleaved into two subunits (residues 30 to 278 and 279 to 402) Sphingosinicella xenopeptidilytica

Purification (Commentary)

Purification (Comment) Organism
-
 Sphingosinicella xenopeptidilytica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
 pH 7.0, 30°C Sphingosinicella xenopeptidilytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-homoAla-beta-homoLeu + H2O
-
 Sphingosinicella xenopeptidilytica beta-homoAla + beta-homoLeu
-
 ?
beta-homoAla-beta-homoLeu + H2O
-
 Sphingosinicella xenopeptidilytica 3-2W4 beta-homoAla + beta-homoLeu
-
 ?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
-
 Sphingosinicella xenopeptidilytica beta-homoVal + beta-homoAla-beta-homoLeu
-
 ?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
-
 Sphingosinicella xenopeptidilytica 3-2W4 beta-homoVal + beta-homoAla-beta-homoLeu
-
 ?

Subunits

Subunits Comment Organism
? x * 12000 + x * 27000, SDS-PAGE Sphingosinicella xenopeptidilytica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Sphingosinicella xenopeptidilytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Sphingosinicella xenopeptidilytica

General Information

General Information Comment Organism
metabolism Sphingosinicella xenopeptidilytica strain 3-2W4 is able to utilize the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu as sole carbon and energy sources Sphingosinicella xenopeptidilytica