Any feedback?
Literature summary for 3.4.11.24 extracted from
- Mechanistic role of each metal ion in Streptomyces dinuclear aminopeptidase: PEPTIDE hydrolysis and 7x10(10)-fold rate enhancement of phosphodiester hydrolysis (2010), J. Inorg. Biochem., 104, 19-29.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fluoride | - |
Streptomyces griseus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00229 | - |
Leu-4-nitroanilide | in presence of Ni-Ni-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.00242 | - |
Leu-4-nitroanilide | in presence of Mn-Ni-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.093 | - |
Leu-4-nitroanilide | in presence of Co-Co-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.15 | - |
Leu-4-nitroanilide | in presence of Mn-Co-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.19 | - |
Leu-4-nitroanilide | in presence of Mn-Cd-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.213 | - |
Leu-4-nitroanilide | in presence of Cd-Cd-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.88 | - |
Leu-4-nitroanilide | in presence of Mn-Mn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 3.27 | - |
Leu-4-nitroanilide | in presence of Zn-Zn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 3.31 | - |
Leu-4-nitroanilide | in presence of Mn-Zn-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 3.8 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Zn-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 3.9 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Co-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 4.5 | - |
bis(4-nitrophenyl) phosphate | in presence of Zn-Zn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 9.5 | - |
bis(4-nitrophenyl) phosphate | in presence of Co-Co-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 9.7 | - |
bis(4-nitrophenyl) phosphate | in presence of Cd-Cd-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 10.6 | - |
bis(4-nitrophenyl) phosphate | in presence of Ni-Ni-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 11 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Cd-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 12.3 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Mn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 12.8 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Ni-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces griseus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE Sephacel column | Streptomyces griseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bis(4-nitrophenyl) phosphate + H2O | assay at pH 8.0, 30°C | Streptomyces griseus | ? | - |
? | |
| Leu-4-nitroanilide + H2O | assay at pH 8.0, 30°C | Streptomyces griseus | Leu + 4-nitroaniline | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dinuclear aminopeptidase | - |
Streptomyces griseus |
| SGAP | - |
Streptomyces griseus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Streptomyces griseus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0064 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Ni-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.01 | - |
bis(4-nitrophenyl) phosphate | in presence of Ni-Ni-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.016 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Cd-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.043 | - |
bis(4-nitrophenyl) phosphate | in presence of Cd-Cd-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.0433 | - |
Leu-4-nitroanilide | in presence of Ni-Ni-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.0505 | - |
Leu-4-nitroanilide | in presence of Mn-Ni-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.081 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Mn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.087 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Co-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.1 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Zn-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.45 | - |
bis(4-nitrophenyl) phosphate | in presence of Zn-Zn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.74 | - |
bis(4-nitrophenyl) phosphate | in presence of Co-Co-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 1.5 | - |
Leu-4-nitroanilide | in presence of Mn-Cd-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 1.68 | - |
Leu-4-nitroanilide | in presence of Cd-Cd-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 27.5 | - |
Leu-4-nitroanilide | in presence of Mn-Mn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 37.4 | - |
Leu-4-nitroanilide | in presence of Mn-Co-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 41 | - |
Leu-4-nitroanilide | in presence of Co-Co-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 92.8 | - |
Leu-4-nitroanilide | in presence of Mn-Zn-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 101 | - |
Leu-4-nitroanilide | in presence of Zn-Zn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Streptomyces griseus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
fluoride | substrate Leu-4-nitroanilide, in presence of Mn-Mn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 17 | - |
fluoride | substrate Leu-4-nitroanilide, in presence of Mn-Co-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 28 | - |
fluoride | substrate Leu-4-nitroanilide, in presence of Co-Co-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 70 | - |
fluoride | substrate Leu-4-nitroanilide, in presence of Mn-Zn-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 75 | - |
fluoride | substrate Leu-4-nitroanilide, in presence of Mn-Ni-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 82 | - |
fluoride | substrate Leu-4-nitroanilide, in presence of Ni-Ni-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 108 | - |
fluoride | substrate Leu-4-nitroanilide, in presence of Zn-Zn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Ni-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.00094 | - |
bis(4-nitrophenyl) phosphate | in presence of NNi-Ni-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.00142 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Cd-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.0044 | - |
bis(4-nitrophenyl) phosphate | in presence of Cd-Cd-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.00467 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Mn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.022 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Co-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.027 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Zn-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.078 | - |
bis(4-nitrophenyl) phosphate | in presence of Co-Co-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 0.1 | - |
bis(4-nitrophenyl) phosphate | in presence of Zn-Zn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 7.89 | - |
Leu-4-nitroanilide | in presence of Cd-Cd-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 7.89 | - |
Leu-4-nitroanilide | in presence of Mn-Cd-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 18.9 | - |
Leu-4-nitroanilide | in presence of Ni-Ni-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 20.9 | - |
Leu-4-nitroanilide | in presence of Mn-Ni-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 28 | - |
Leu-4-nitroanilide | in presence of Mn-Zn-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 30.9 | - |
Leu-4-nitroanilide | in presence of Zn-Zn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 31.4 | - |
Leu-4-nitroanilide | in presence of Mn-Mn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
| 249 | - |
Leu-4-nitroanilide | in presence of Mn-Co-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
| 441 | - |
Leu-4-nitroanilide | in presence of Co-Co-homo-dinuclear aminopeptidase | Streptomyces griseus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
