Literature summary for 3.4.11.24 extracted from

Herrera-Camacho, I.; Morales-Monterrosas, R.; Quiroz-Alvarez, R.
Aminopeptidase yscCo-II: a new cobalt-dependent aminopeptidase from yeast-purification and biochemical characterization (2000), Yeast, 16, 219-229.

Search for more literature for 3.4.11.24

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	1 mM, 91% inhibition	Saccharomyces cerevisiae
antipain	0.01 mg/ml, 93% inhibition	Saccharomyces cerevisiae
bestatin	0.004 mg/ml, 94% inhibition	Saccharomyces cerevisiae
Chloroquine	5 mM, 79% inhibition	Saccharomyces cerevisiae
CrCl2	1 mM, 83% inhibition in presence of 1 mM CoCl2	Saccharomyces cerevisiae
CuCl2	1 mM, 96% inhibition in presence of 1 mM CoCl2	Saccharomyces cerevisiae
EDTA	5 mM, 90% inhibition	Saccharomyces cerevisiae
HgCl2	5 mM, 98% inhibition in presence of 1 mM CoCl2	Saccharomyces cerevisiae
Leu-hydroxamate	1 mM, 73% inhibition	Saccharomyces cerevisiae
leupeptin	0.01 mg/ml, 51% inhibition	Saccharomyces cerevisiae
Lys-hydroxamate	1 mM, 81% inhibition	Saccharomyces cerevisiae
MgSO4	1 mM, 24% inhibition in presence of 1 mM CoCl2	Saccharomyces cerevisiae
MnSO4	1 mM, 26% inhibition in presence of 1 mM CoCl2	Saccharomyces cerevisiae
NiSO4	1 mM, 40% inhibition in presence of 1 mM CoCl2	Saccharomyces cerevisiae
nitrilotriacetic acid	5 mM, 95% inhibition	Saccharomyces cerevisiae
p-hydroxymercuribenzoate	5 mM, 90% inhibition	Saccharomyces cerevisiae
phenylmethylsulfonyl fluoride	1 mM, 95% inhibition	Saccharomyces cerevisiae
tosyl-lysine chloromethyl ketone	5 mM, 94% inhibition	Saccharomyces cerevisiae
ZnCl2	1 mM, 70% inhibition in presence of 1 mM CoCl2	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.16	-	Lys-4-nitroanilide	37°C, 0.5 mM CoCl2	Saccharomyces cerevisiae
0.16	-	Arg-4-nitroanilide	37°C, 0.5 mM CoCl2	Saccharomyces cerevisiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	activity is strictly dependent on, maximal activity at 0.5 mM CoCl2	Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	-	6 * 48000, SDS-PAGE	Saccharomyces cerevisiae
290000	-	gel filtration	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4.05	-	-	Saccharomyces cerevisiae

Storage Stability

Storage Stability	Organism
4°C, 50% loss of activity after 4 days, 80% loss of activity after 8 days. Remains active for 2 months, when concentrated and kept in a suspension with 4 M ammonium sulfate in 20 mM MOPS/Tris, pH 7	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Arg-4-nitroanilide + H2O	105.2% of the activity with Lys-4-nitroanilide	Saccharomyces cerevisiae	Arg + 4-nitroaniline	-	?
Glu-4-nitroanilide + H2O	5.4% of the activity with Lys-4-nitroanilide	Saccharomyces cerevisiae	Glu + 4-nitroaniline	-	?
Leu-4-nitroanilide + H2O	12.2% of the activity with Lys-4-nitroanilide	Saccharomyces cerevisiae	Leu + 4-nitroaniline	-	?
Lys-4-nitroanilide + H2O	-	Saccharomyces cerevisiae	Lys + 4-nitroaniline	-	?
Pro-4-nitroanilide + H2O	11.7% of the activity with Lys-4-nitroanilide	Saccharomyces cerevisiae	Pro + 4-nitroaniline	-	?

Subunits

Subunits	Comment	Organism
hexamer	6 * 48000, SDS-PAGE	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
aminopeptidase yscCo-II	-	Saccharomyces cerevisiae
APCo-II	-	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Saccharomyces cerevisiae

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	8	pH 6: about 55% of maximal activity, pH 11: about 35% of maximal activity	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)