Literature summary for 3.4.11.24 extracted from

Fundoiano-Hershcovitz, Y.; Rabinovitch, L.; Langut, Y.; Reiland, V.; Shoham, G.; Shoham, Y.
Identification of the catalytic residues in the double-zinc aminopeptidase from Streptomyces griseus (2004), FEBS Lett., 571, 192-196.

Search for more literature for 3.4.11.24

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination, subcloning and expression of the recombinant enzyme possessing a Asp70 and Asp184 residues in Escherichia coli strain BL21(DE3), the soluble enzyme is inducible by growth on 1 M sorbitol, optimization of the expression method	Streptomyces griseus

Protein Variants

Protein Variants	Comment	Organism
E131	site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme	Streptomyces griseus
Y246	site-directed mutagenesis, the mutant enzyme shows highly reduced activity compared to the wild-type enzyme	Streptomyces griseus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	-	Streptomyces griseus
Zn2+	the enzyme is a double-zinc aminopeptidase	Streptomyces griseus

Organism

Organism	UniProt	Comment	Textmining
Streptomyces griseus	-	DSM 40855	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant soluble wild-type and mutant enzymes 28fold from Escherichia coli strain BL21(DE3) by heat treatment for 20 min at 50°C and anion exchange chromatography	Streptomyces griseus

Reaction

Reaction	Comment	Organism	Reaction ID
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues	catalytic pathway and reaction mechanism, catalytic residues are Glu131 and Tyr246, Tyr246 is involved in stabilization of the reaction transition state intermediate, also residue Glu151 is involved	Streptomyces griseus	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
830	-	purified recombinant wild-type enzyme	Streptomyces griseus

Synonyms

Synonyms	Comment	Organism
double-zinc aminopeptidase	-	Streptomyces griseus
SGAP	-	Streptomyces griseus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Streptomyces griseus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	20 min, stable	Streptomyces griseus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Streptomyces griseus

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Release 2023.1 (January 2023)