Literature summary for 3.4.11.24 extracted from

Ercan, A.; Park, H.I.; Ming, L.J.
Remarkable enhancement of the hydrolyses of phosphoesters by dinuclear centers: Streptomyces aminopeptidase as a 'natural model system' (2000), Chem. Commun. (Camb.), 1, 2501-2502.

No PubMed abstract available

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.4	-	4-nitrophenyl phenylphosphonate	pH 8.0, 50°C, Cd2+-enzyme	Streptomyces sp.
3	-	4-nitrophenyl phenylphosphonate	pH 8.0, 50°C, Ni2+-enzyme	Streptomyces sp.
4.5	-	bis(4-nitrophenyl) phosphate	pH 8.0, 30°C, Zn2+-enzyme	Streptomyces sp.
4.9	-	4-nitrophenyl phenylphosphonate	pH 8.0, 50°C, Mn2+-enzyme	Streptomyces sp.
7.9	-	4-nitrophenyl phenylphosphonate	pH 8.0, 50°C, Co2+-enzyme	Streptomyces sp.
9.5	-	bis(4-nitrophenyl) phosphate	pH 8.0, 30°C, Co2+-enzyme	Streptomyces sp.
9.7	-	bis(4-nitrophenyl) phosphate	pH 8.0, 30°C, Cd2+-enzyme	Streptomyces sp.
10.6	-	bis(4-nitrophenyl) phosphate	pH 8.0, 30°C, Ni2+-enzyme	Streptomyces sp.
12	-	bis(4-nitrophenyl) phosphate	pH 8.0, 30°C, Mn2+-enzyme	Streptomyces sp.
14.9	-	4-nitrophenyl phenylphosphonate	pH 8.0, 50°C, Zn2+-enzyme	Streptomyces sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cd2+	dinuclear metal-enzyme derivative, structure	Streptomyces sp.
Co2+	dinuclear metal-enzyme derivative, structure	Streptomyces sp.
Mn2+	dinuclear metal-enzyme derivative, structure	Streptomyces sp.
additional information	the enzyme possesses dinuclear metal cluster	Streptomyces sp.
Ni2+	dinuclear metal-enzyme derivative, structure	Streptomyces sp.

Organism

Organism	UniProt	Comment	Textmining
Streptomyces sp.	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0016	-	Ni2+-enzyme, substrate 4-nitrophenyl phenylphosphonate	Streptomyces sp.
0.0016	-	Ni2+-enzyme, substrate bis(4-nitrophenyl) phosphate	Streptomyces sp.
0.0017	-	Zn2+-enzyme, substrate 4-nitrophenyl phenylphosphonate	Streptomyces sp.
0.0033	-	Mn2+-enzyme, substrate 4-nitrophenyl phenylphosphonate	Streptomyces sp.
0.0048	-	Co2+-enzyme, substrate 4-nitrophenyl phenylphosphonate	Streptomyces sp.
0.0078	-	Cd2+-enzyme, substrate bis(4-nitrophenyl) phosphate	Streptomyces sp.
0.0097	-	Cd2+-enzyme, substrate 4-nitrophenyl phenylphosphonate	Streptomyces sp.
0.031	-	Mn2+-enzyme, substrate bis(4-nitrophenyl) phosphate	Streptomyces sp.
0.136	-	Co2+-enzyme, substrate bis(4-nitrophenyl) phosphate	Streptomyces sp.
0.158	-	Zn2+-enzyme, substrate bis(4-nitrophenyl) phosphate	Streptomyces sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl phenylphosphonate + H2O	-	Streptomyces sp.	4-nitrophenol + phenylphosphonate	-	?
bis(4-nitrophenyl) phosphate + H2O	-	Streptomyces sp.	4-nitrophenol + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
Streptomyces aminopeptidase	-	Streptomyces sp.
Streptomyces dinuclear aminopeptidase	-	Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at, substrate bis(4-nitrophenyl) phosphate	Streptomyces sp.
50	-	assay at, substrate 4-nitrophenyl phenylphosphonate	Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0033	-	4-nitrophenyl phenylphosphonate	pH 8.0, 50°C, Ni2+-enzyme	Streptomyces sp.
0.01	-	bis(4-nitrophenyl) phosphate	pH 8.0, 30°C, Ni2+-enzyme	Streptomyces sp.
0.01	-	4-nitrophenyl phenylphosphonate	pH 8.0, 50°C, Mn2+-enzyme	Streptomyces sp.
0.014	-	4-nitrophenyl phenylphosphonate	pH 8.0, 50°C, Zn2+-enzyme	Streptomyces sp.
0.017	-	4-nitrophenyl phenylphosphonate	pH 8.0, 50°C, Cd2+-enzyme	Streptomyces sp.
0.022	-	4-nitrophenyl phenylphosphonate	pH 8.0, 50°C, Co2+-enzyme	Streptomyces sp.
0.043	-	bis(4-nitrophenyl) phosphate	pH 8.0, 30°C, Cd2+-enzyme	Streptomyces sp.
0.21	-	bis(4-nitrophenyl) phosphate	pH 8.0, 30°C, Mn2+-enzyme	Streptomyces sp.
0.45	-	bis(4-nitrophenyl) phosphate	pH 8.0, 30°C, Zn2+-enzyme	Streptomyces sp.
0.74	-	bis(4-nitrophenyl) phosphate	pH 8.0, 30°C, Co2+-enzyme	Streptomyces sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Streptomyces sp.

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Release 2023.1 (January 2023)