Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Streptomyces septatus |
Protein Variants | Comment | Organism |
---|---|---|
F221A | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Streptomyces septatus |
F221G | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Streptomyces septatus |
F221S | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Streptomyces septatus |
additional information | alteration of leucine aminopeptidase to phenylalanine aminopeptidase by site-directed mutagenesis | Streptomyces septatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of recombinant wild-type and F221 mutant enzymes | Streptomyces septatus | |
0.25 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant mutant F221I | Streptomyces septatus | |
0.34 | - |
L-Phe-4-nitroanilide | pH 8.0, 37°C, recombinant wild-type enzyme | Streptomyces septatus | |
0.36 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant wild-type enzyme | Streptomyces septatus | |
0.44 | - |
L-Phe-4-nitroanilide | pH 8.0, 37°C, recombinant mutant F221A | Streptomyces septatus | |
0.68 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant mutant F221A | Streptomyces septatus | |
1.85 | - |
L-Phe-4-nitroanilide | pH 8.0, 37°C, recombinant mutant F221I | Streptomyces septatus | |
6.9 | - |
L-Phe-O-methyl ester | pH 8.0, 37°C, recombinant wild-type enzyme | Streptomyces septatus | |
6.98 | - |
L-Phe-NH2 | pH 8.0, 37°C, recombinant wild-type enzyme | Streptomyces septatus | |
7.68 | - |
L-Phe-NH2 | pH 8.0, 37°C, recombinant mutant F221A | Streptomyces septatus | |
8.31 | - |
L-Leu-NH2 | pH 8.0, 37°C, recombinant mutant F221I | Streptomyces septatus | |
12.8 | - |
L-Phe-O-methyl ester | pH 8.0, 37°C, recombinant mutant F221A | Streptomyces septatus | |
15.6 | - |
L-Leu-NH2 | pH 8.0, 37°C, recombinant wild-type enzyme | Streptomyces septatus | |
15.8 | - |
L-Leu-O-methyl ester | pH 8.0, 37°C, recombinant wild-type enzyme | Streptomyces septatus | |
16.1 | - |
L-Leu-O-methyl ester | pH 8.0, 37°C, recombinant mutant F221I | Streptomyces septatus | |
24.2 | - |
L-Phe-NH2 | pH 8.0, 37°C, recombinant mutant F221I | Streptomyces septatus | |
42.7 | - |
L-Leu-NH2 | pH 8.0, 37°C, recombinant mutant F221A | Streptomyces septatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces septatus | Q75V72 | gene Ssap | - |
Streptomyces septatus TH-2 | Q75V72 | gene Ssap | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) | Streptomyces septatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Streptomyces septatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Leu-4-nitroanilide + H2O | preferred substrate of the wild-type enzyme | Streptomyces septatus | L-Leu + 4-nitroaniline | - |
? | |
L-Leu-4-nitroanilide + H2O | preferred substrate of the wild-type enzyme | Streptomyces septatus TH-2 | L-Leu + 4-nitroaniline | - |
? | |
L-Leu-NH2 + H2O | - |
Streptomyces septatus | L-Leu + NH3 | - |
? | |
L-Leu-NH2 + H2O | - |
Streptomyces septatus TH-2 | L-Leu + NH3 | - |
? | |
L-Leu-O-methyl ester + H2O | - |
Streptomyces septatus | L-Leu + methanol | - |
? | |
L-Leu-O-methyl ester + H2O | - |
Streptomyces septatus TH-2 | L-Leu + methanol | - |
? | |
L-Phe-4-nitroanilide + H2O | - |
Streptomyces septatus | L-Phe + 4-nitroaniline | - |
? | |
L-Phe-4-nitroanilide + H2O | - |
Streptomyces septatus TH-2 | L-Phe + 4-nitroaniline | - |
? | |
L-Phe-NH2 + H2O | - |
Streptomyces septatus | L-Phe + NH3 | - |
? | |
L-Phe-O-methyl ester + H2O | - |
Streptomyces septatus | L-Phe + methanol | - |
? | |
additional information | substrate specificities of recombinant wild-type and F221 mutant enzymes, overview | Streptomyces septatus | ? | - |
? | |
additional information | substrate specificities of recombinant wild-type and F221 mutant enzymes, overview | Streptomyces septatus TH-2 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
leucine aminopeptidase | - |
Streptomyces septatus |
SSAP | - |
Streptomyces septatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptomyces septatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.64 | - |
L-Phe-O-methyl ester | pH 8.0, 37°C, recombinant wild-type enzyme | Streptomyces septatus | |
10.7 | - |
L-Phe-NH2 | pH 8.0, 37°C, recombinant mutant F221I | Streptomyces septatus | |
17.6 | - |
L-Phe-4-nitroanilide | pH 8.0, 37°C, recombinant wild-type enzyme | Streptomyces septatus | |
19.3 | - |
L-Phe-4-nitroanilide | pH 8.0, 37°C, recombinant mutant F221I | Streptomyces septatus | |
31.7 | - |
L-Leu-NH2 | pH 8.0, 37°C, recombinant mutant F221A | Streptomyces septatus | |
34 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant mutant F221A | Streptomyces septatus | |
43.8 | - |
L-Phe-NH2 | pH 8.0, 37°C, recombinant wild-type enzyme | Streptomyces septatus | |
54.3 | - |
L-Phe-O-methyl ester | pH 8.0, 37°C, recombinant wild-type enzyme | Streptomyces septatus | |
64.2 | - |
L-Leu-O-methyl ester | pH 8.0, 37°C, recombinant wild-type enzyme | Streptomyces septatus | |
65.9 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant wild-type enzyme | Streptomyces septatus | |
104 | - |
L-Leu-NH2 | pH 8.0, 37°C, recombinant wild-type enzyme | Streptomyces septatus | |
108 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant mutant F221I | Streptomyces septatus | |
115 | - |
L-Phe-NH2 | pH 8.0, 37°C, recombinant mutant F221A | Streptomyces septatus | |
142 | - |
L-Leu-O-methyl ester | pH 8.0, 37°C, recombinant wild-type enzyme | Streptomyces septatus | |
155 | - |
L-Leu-NH2 | pH 8.0, 37°C, recombinant mutant F221I | Streptomyces septatus | |
169 | - |
L-Leu-O-methyl ester | pH 8.0, 37°C, recombinant mutant F221I | Streptomyces septatus | |
224 | - |
L-Phe-O-methyl ester | pH 8.0, 37°C, recombinant mutant F221A | Streptomyces septatus | |
229 | - |
L-Phe-4-nitroanilide | pH 8.0, 37°C, recombinant mutant F221A | Streptomyces septatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces septatus |