Literature summary for 3.4.11.24 extracted from

Reiland, V.; Gilboa, R.; Spungin-Bialik, A.; Schomburg, D.; Shoham, Y.; Blumberg, S.; Shoham, G.
Binding of inhibitory aromatic amino acids to Streptomyces griseus aminopeptidase (2004), Acta Crystallogr. Sect. D, 60, 1738-1746.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme in complex with tryptophan or 4-iodo-L-phenylalanine, hanging drop vapour diffusion method, 18 mg/ml protein in 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 6 mM CaCl2, and 100 mM tryptophan or 2 mM 4-iodo-L-phenylalanine, equal volumes of protein and reservoir solution are mixed, the latter containing 18% w/v PEG 4000 and 0.1 M sodium acetate, pH 5.5, equilibration against 1 ml reservoir solution for 1 day, microseeding, 3-4 days, X-ray diffraction structure determination and analysis at 1.3 A resolution	Streptomyces griseus

Crystallization (Comment)

Organism

purified enzyme in complex with tryptophan or 4-iodo-L-phenylalanine, hanging drop vapour diffusion method, 18 mg/ml protein in 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 6 mM CaCl2, and 100 mM tryptophan or 2 mM 4-iodo-L-phenylalanine, equal volumes of protein and reservoir solution are mixed, the latter containing 18% w/v PEG 4000 and 0.1 M sodium acetate, pH 5.5, equilibration against 1 ml reservoir solution for 1 day, microseeding, 3-4 days, X-ray diffraction structure determination and analysis at 1.3 A resolution

Streptomyces griseus

Inhibitors

Inhibitors	Comment	Organism	Structure
4-iodo-L-phenylalanine	weak inhibition, binds at the active site via the two zinc ions displacing the metal ions, binding structure analysis	Streptomyces griseus
L-tryptophan	weak inhibition, binds at the active site via the two zinc ions displacing the metal ions, binding structure analysis	Streptomyces griseus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	the enzyme is a double-zinc aminopeptidase, the metal ions are bound at the active site, binding structure analysis	Streptomyces griseus

Organism

Organism	UniProt	Comment	Textmining
Streptomyces griseus	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues	raction mechanism, catalytic residues are Glu131 and Tyr246, residues Arg202 and Asp160 stabilize the reaction intermediate together with Glu131	Streptomyces griseus	a

Synonyms

Synonyms	Comment	Organism
SGAP	-	Streptomyces griseus
Streptomyces griseus aminopeptidase	-	Streptomyces griseus