Literature summary for 3.4.11.24 extracted from

Greenblatt, H.M.; Almog, O.; Maras, B.; Spungin-Bialik, A.; Barra, D.; Blumberg, S.; Shoham, G.
Streptomyces griseus aminopeptidase: X-ray crystallographic structure at 1.75 A resolution (1997), J. Mol. Biol., 265, 620-636.

Search for more literature for 3.4.11.24

Crystallization (Commentary)

Crystallization (Comment)	Organism
protein with or without bound Zn2+ or replaced with Hg2+, hanging drop vapour diffusion method, 20 mg/ml purified enzyme in 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 1 mM CaCl2, plus an equal volume of sodium acetate buffer at pH 5.0 to 6.0, 16-20% w/v polyethylene glycol 4000, suspended over 1 ml reservoir solution of sodium acetate, pH 5.0-6.0, 16-20% PEG 4000, 4-5 weeks to full size crystals, X-ray diffraction structure determination at 2.1 to 1.75 A resolution and analysis	Streptomyces griseus

Crystallization (Comment)

Organism

protein with or without bound Zn2+ or replaced with Hg2+, hanging drop vapour diffusion method, 20 mg/ml purified enzyme in 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 1 mM CaCl2, plus an equal volume of sodium acetate buffer at pH 5.0 to 6.0, 16-20% w/v polyethylene glycol 4000, suspended over 1 ml reservoir solution of sodium acetate, pH 5.0-6.0, 16-20% PEG 4000, 4-5 weeks to full size crystals, X-ray diffraction structure determination at 2.1 to 1.75 A resolution and analysis

Streptomyces griseus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	location of binding site is in about 25 A distance from the zinc binding site, determination of structural environment	Streptomyces griseus
Zn2+	2 mol zinc per mol of enzyme, tightly bound at the active site in a distance of 3.6 A of each other, determination of structural environment	Streptomyces griseus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
peptide + H2O	Streptomyces griseus	-	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces griseus	-	purified enzyme from commercial product	-

Reaction

Reaction	Comment	Organism	Reaction ID
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues	M161 is involved in substrate binding at the active site cleft	Streptomyces griseus	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial product	protease type XIV i.e. pronase E, a protease mixture form Streptomyces griseus	Streptomyces griseus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
peptide + H2O	-	Streptomyces griseus	?	-	?

Synonyms

Synonyms	Comment	Organism
M28.003	Merops-ID	Streptomyces griseus
SGAP	-	Streptomyces griseus