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Literature summary for 3.4.11.24 extracted from

  • Papir, G.; Spungin-Bialik, A.; Ben-Meir, D.; Fudim, E.; Gilboa, R.; Greenblatt, H.M.; Shoham, G.; Lessel, U.; Schomburg, D.; Ashkenazi, R.; Blumberg, S.
    Inhibition of Streptomyces griseus aminopeptidase and effects of calcium ions on catalysis and binding--comparisons with the homologous enzyme Aeromonas proteolytica aminopeptidase (1998), Eur. J. Biochem., 258, 313-319.
    View publication on PubMed
Search for more literature for 3.4.11.24

General Stability

General Stability Organism
Ca2+ stabilizes Streptomyces griseus

Inhibitors

Inhibitors Comment Organism Structure
amastatin
-
 Streptomyces griseus
bestatin
-
 Streptomyces griseus
D-phenylalanine
-
 Streptomyces griseus
L-alanine
-
 Streptomyces griseus
L-arginine
-
 Streptomyces griseus
L-aspartate
-
 Streptomyces griseus
L-leucine
-
 Streptomyces griseus
L-leucine chloromethyl ketone
-
 Streptomyces griseus
L-methionine
-
 Streptomyces griseus
L-phenylalanine
-
 Streptomyces griseus
L-phenylalanine chloromethyl ketone
-
 Streptomyces griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Streptomyces griseus
0.55
-
 L-leucine-4-nitroanilide pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
0.79
-
 L-valine-4-nitroanilide pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
2.15
-
 L-valine-4-nitroanilide pH 8.0, 30°C, in absence of Ca2+ Streptomyces griseus
2.97
-
 L-leucine-4-nitroanilide pH 8.0, 30°C, in absence of Ca2+ Streptomyces griseus
3.84
-
 L-alanine-4-nitroanilide pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
4.81
-
 L-alanine-4-nitroanilide pH 8.0, 30°C, in absence of Ca2+ Streptomyces griseus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates, enhances stability of the enzyme, modulates the enzyme activity and affinity towards substrates and inhibitors in a structure-dependent manner, binding structure Streptomyces griseus
Zn2+ 2 mol zinc per mol of enzyme, binding structure Streptomyces griseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000 29730 native PAGE and mass spectrometry Streptomyces griseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptide + H2O Streptomyces griseus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces griseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from commercial product Streptomyces griseus

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation protease type XIV i.e. pronase E, a protease mixture form Streptomyces griseus Streptomyces griseus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Streptomyces griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine-4-nitroanilide + H2O
-
 Streptomyces griseus L-alanine + 4-nitroaniline
-
 ?
L-leucine-4-nitroanilide + H2O best of the amino acid 4-nitroanilide substrates Streptomyces griseus L-leucine + 4-nitroaniline
-
 ?
L-valine-4-nitroanilide + H2O
-
 Streptomyces griseus L-valine + 4-nitroaniline
-
 ?
peptide + H2O
-
 Streptomyces griseus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 29728-29731, mass spectrometry Streptomyces griseus

Synonyms

Synonyms Comment Organism
M28.003 Merops-ID Streptomyces griseus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 stable at Streptomyces griseus
75
-
 rate constant for inactivation Kin is 0.00028/s in absence of Ca2+, and 0.000025/s in presence of Ca2+ Streptomyces griseus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
 L-valine-4-nitroanilide pH 8.0, 30°C, in absence of Ca2+ Streptomyces griseus
0.26
-
 L-valine-4-nitroanilide pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
0.47
-
 L-alanine-4-nitroanilide pH 8.0, 30°C, in absence of Ca2+ Streptomyces griseus
1.31
-
 L-leucine-4-nitroanilide pH 8.0, 30°C, in absence of Ca2+ Streptomyces griseus
1.81
-
 L-alanine-4-nitroanilide pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
2.94
-
 L-alanine-4-nitroanilide pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000016
-
 amastatin pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
0.0005
-
 L-leucine chloromethyl ketone pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
0.00055
-
 amastatin pH 8.0, 30°C, in absence of Ca2+ Streptomyces griseus
0.0019
-
 L-phenylalanine chloromethyl ketone pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
0.0026
-
 bestatin pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
0.0053
-
 L-phenylalanine chloromethyl ketone pH 8.0, 30°C, in absence of Ca2+ Streptomyces griseus
0.0054
-
 bestatin pH 8.0, 30°C, in absence of Ca2+ Streptomyces griseus
0.0055
-
 L-leucine chloromethyl ketone pH 8.0, 30°C, in absence of Ca2+ Streptomyces griseus
8.7
-
 L-methionine pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
9.1
-
 L-methionine pH 6.5, 30°C, in presence of Ca2+ Streptomyces griseus
12.4
-
 L-leucine pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
12.7
-
 L-phenylalanine pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
12.8
-
 L-leucine pH 6.5, 30°C, in presence of Ca2+ Streptomyces griseus
36.6
-
 D-phenylalanine pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus