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Literature summary for 3.4.11.24 extracted from
- Streptomyces griseus aminopeptidase is a calcium-activated zinc metalloprotein. Purification and properties of the enzyme (1989), Eur. J. Biochem., 183, 471-477.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 50% inhibition at pI 4.9 | Streptomyces griseus |  |
| EDTA | complete inhibition, activity cannot be completely restored by addition of 1 mM CaCl2 alone but together with 0.0001 mM ZnCl2 by 90% | Streptomyces griseus | |
| EGTA | complete inhibition, activity can be completely restored by addition of 1 mM CaCl2 | Streptomyces griseus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates about 6fold, non-cooperative binding | Streptomyces griseus | |
| Zn2+ | metalloprotease, dependent on | Streptomyces griseus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzymes are retarded on the Superose gel | Streptomyces griseus |
| 21000 | - |
API, gel filtration | Streptomyces griseus |
| 22500 | - |
APII, gel filtration | Streptomyces griseus |
| 33000 | - |
1 * 33000, API, SDS-PAGE, 1 * 34000, APII, SDS-PAGE | Streptomyces griseus |
| 34000 | - |
1 * 33000, API, SDS-PAGE, 1 * 34000, APII, SDS-PAGE | Streptomyces griseus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| peptide + H2O | Streptomyces griseus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces griseus | P80561 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from commercial product, 2 peaks API and APII, endopeptidase is eliminated | Streptomyces griseus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | protease type XIV i.e. pronase E, a protease mixture form Streptomyces griseus | Streptomyces griseus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 306 | - |
purified API | Streptomyces griseus |
| 317 | - |
purified APII | Streptomyces griseus |
| 461 | - |
purified API in presence of 1 mM CaCl2 | Streptomyces griseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-leucine-4-nitroanilide + H2O | - |
Streptomyces griseus | L-leucine + 4-nitroaniline | - |
? | |
| peptide + H2O | - |
Streptomyces griseus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 33000, API, SDS-PAGE, 1 * 34000, APII, SDS-PAGE | Streptomyces griseus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AP | - |
Streptomyces griseus |
| M28.003 | Merops-ID | Streptomyces griseus |
| SGAP | - |
Streptomyces griseus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Streptomyces griseus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 69 | - |
stable during heating for 5 h at 69°C as part of the purification process | Streptomyces griseus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Streptomyces griseus |
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