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Literature summary for 3.4.11.23 extracted from
- Salmonella enterica serovar Typhimurium peptidase B is a leucyl aminopeptidase with specificity for acidic amino acids (2000), J. Bacteriol., 182, 3383-3393.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| divalent cations | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| KCl | stimulation, optimal at 50 mM | Salmonella enterica subsp. enterica serovar Typhimurium |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence determination, overexpression from plasmid, Salmonella typhimurium strain TN5179 | Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K310V | site-directed mutagenesis, more than 200fold reduction in the rate of Asp-Leu hydrolysis with no decrease in the rate of Leu-Leu hydrolysis | Salmonella enterica subsp. enterica serovar Typhimurium |
| R402W | site-directed mutagenesis, reduction in the rate of Asp-Leu hydrolysis with no decrease in the rate of Leu-Leu hydrolysis | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| bestatin | complete inhibition at 1 mM, 37°C, after 20 min | Salmonella enterica subsp. enterica serovar Typhimurium | |
| EDTA | reversible by divalent cations | Salmonella enterica subsp. enterica serovar Typhimurium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km-values for diverse substrates, kinetics | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.5 | - |
Asp-Leu-Gly | 37°C, pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.9 | - |
Glu-Leu | 37°C, pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4 | - |
Leu-Leu | 37°C, pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
6 * 45000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
| 270000 | - |
gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Leu + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Ala + Leu | - |
? | |
| Asn-Leu + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Asn + Leu | - |
? | |
| Asp-Leu + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | best substrate | Asp + Leu | - |
? | |
| Cys-Leu + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Cys + Leu | - |
? | |
| Glu-Leu + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Glu + Leu | - |
? | |
| His-Leu + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
His + Leu | - |
? | |
| Leu-Cys + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Leu + Cys | - |
? | |
| Leu-His + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Leu + His | - |
? | |
| Leu-Ile + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Leu + Ile | - |
? | |
| Leu-Leu + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Leu + Leu | - |
? | |
| Leu-Met + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Leu + Met | - |
? | |
| Leu-Phe + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Leu + Phe | - |
? | |
| Leu-Tyr + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Leu + Tyr | - |
? | |
| Leu-Xaa + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | Xaa is an amino acid, substrate specificity, overview | Leu + Xaa | - |
? | |
| Leu-Xaa + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | lower activity compared to substrate Asp-Leu | Leu + Xaa | - |
? | |
| Met-Leu + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Met + Leu | - |
? | |
| Ser-Leu + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Ser + Leu | - |
? | |
| Thr-Leu + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Thr + Leu | - |
? | |
| Xaa-peptide + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | substrate specificity, overview | Xaa + peptide | - |
? | |
| Xaa-peptide + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | Xaa is an amino acid | Xaa + peptide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
gene pepB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from strain TN5179 | Salmonella enterica subsp. enterica serovar Typhimurium |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| release of an N-terminal amino acid, Xaa, from a peptide or arylamide. Xaa is preferably Glu or Asp but may be other amino acids, including Leu, Met, His, Cys and Gln | residue K310 is involved in substrate specificity determination | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
substrate specificity, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
| 190 | - |
purified recombinant enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Leu + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Ala + Leu | - |
? | |
| Ala-Leu + H2O | 3% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Ala + Leu | - |
? | |
| Asn-Leu + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Asn + Leu | - |
? | |
| Asn-Leu + H2O | 23% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Asn + Leu | - |
? | |
| Asp-Ile + H2O | best substrate, 120% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Asp + Ile | - |
? | |
| Asp-Leu + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Asp + Leu | - |
? | |
| Asp-Leu + H2O | best substrate | Salmonella enterica subsp. enterica serovar Typhimurium | Asp + Leu | - |
? | |
| Asp-Leu-Gly + H2O | higher activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Asp + Leu-Gly | - |
? | |
| Asp-Phe + H2O | 105% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Asp + Phe | - |
? | |
| Asp-Xaa + H2O | Xaa is an amino acid, substrate specificity, overview | Salmonella enterica subsp. enterica serovar Typhimurium | Asp + Xaa | - |
? | |
| Cys-Leu + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Cys + Leu | - |
? | |
| Cys-Leu + H2O | 23% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Cys + Leu | - |
? | |
| Gln-Leu + H2O | 9% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Gln + Leu | - |
? | |
| Glu-Leu + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Glu + Leu | - |
? | |
| Glu-Leu + H2O | 74% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Glu + Leu | - |
? | |
| His-Leu + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | His + Leu | - |
? | |
| His-Leu + H2O | 28% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | His + Leu | - |
? | |
| Ile-Leu + H2O | 2% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Ile + Leu | - |
? | |
| Leu-Cys + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Cys | - |
? | |
| Leu-Cys + H2O | 24% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Cys | - |
? | |
| Leu-His + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Leu + His | - |
? | |
| Leu-His + H2O | 9% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Leu + His | - |
? | |
| Leu-Ile + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Ile | - |
? | |
| Leu-Ile + H2O | 59% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Ile | - |
? | |
| Leu-Leu + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Leu | - |
? | |
| Leu-Leu + H2O | 40% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Leu | - |
? | |
| Leu-Met + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Met | - |
? | |
| Leu-Met + H2O | 30% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Met | - |
? | |
| Leu-Phe + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Phe | - |
? | |
| Leu-Phe + H2O | 12% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Phe | - |
? | |
| Leu-Tyr + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Tyr | - |
? | |
| Leu-Tyr + H2O | 37% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Tyr | - |
? | |
| Leu-Xaa + H2O | Xaa is an amino acid, substrate specificity, overview | Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Xaa | - |
? | |
| Leu-Xaa + H2O | lower activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Leu + Xaa | - |
? | |
| Met-Leu + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Met + Leu | - |
? | |
| Met-Leu + H2O | 30% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Met + Leu | - |
? | |
| Phe-Leu + H2O | 7% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Phe + Leu | - |
? | |
| Ser-Leu + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Ser + Leu | - |
? | |
| Ser-Leu + H2O | 2% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Ser + Leu | - |
? | |
| Thr-Leu + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Thr + Leu | - |
? | |
| Thr-Leu + H2O | 3% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Thr + Leu | - |
? | |
| Trp-Leu + H2O | 4% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Trp + Leu | - |
? | |
| Tyr-Leu + H2O | 6% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Tyr + Leu | - |
? | |
| Val-Leu + H2O | 2% activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Val + Leu | - |
? | |
| Xaa-Gly-Gly + H2O | Xaa is an amino acid, substrate specificity, overview | Salmonella enterica subsp. enterica serovar Typhimurium | Xaa + Gly-Gly | - |
? | |
| Xaa-Gly-Gly + H2O | low activity compared to substrate Asp-Leu | Salmonella enterica subsp. enterica serovar Typhimurium | Xaa + Gly-Gly | - |
? | |
| Xaa-Leu + H2O | Xaa is an amino acid, substrate specificity, overview | Salmonella enterica subsp. enterica serovar Typhimurium | Xaa + Leu | - |
? | |
| Xaa-peptide + H2O | substrate specificity, overview | Salmonella enterica subsp. enterica serovar Typhimurium | Xaa + peptide | - |
? | |
| Xaa-peptide + H2O | Xaa is an amino acid | Salmonella enterica subsp. enterica serovar Typhimurium | Xaa + peptide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 45000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
| hexamer | 6 * 46360-46362, mass spectrometry and amino acid sequence determination | Salmonella enterica subsp. enterica serovar Typhimurium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PepB | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
20 min, stable | Salmonella enterica subsp. enterica serovar Typhimurium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | substrate specificity, overview | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 866 | - |
Glu-Leu | 37°C, pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 918 | - |
Leu-Leu | 37°C, pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 950 | - |
Asp-Leu-Gly | 37°C, pH 8.5 | Salmonella enterica subsp. enterica serovar Typhimurium | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9.5 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
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