Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
molecular dynamics simulation of mutants K228R, Q730E, R528R/Q730E. The K528R polymorphism has strong effects on the conformational distribution of ERAP1 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K528R | single nucelotide polymorphism, about 50% of wild-type activtiy with fluorogenic substrate L-leucine-7-amido-4-methylcoumarin. Mutation of position 528 does not appear to affect length selection, but rather affects enzyme activity in a uniform manner | Homo sapiens |
K528R/Q730E | combination of single nucleotide polymorphisms, activity similar to wild-type. The effects of mutations K528R and Q730E are independent and additive | Homo sapiens |
Q730E | single nucelotide polymorphism, about 2000% of wild-type activtiy with fluorogenic substrate L-leucine-7-amido-4-methylcoumarin. Mutation at position 730 leads to an increased preference for smaller peptides | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NZ08 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucine-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | L-leucine + 7-amino-4-methylcoumarin | - |
? | |
LGGGGGGGGGGL + H2O | - |
Homo sapiens | L-leucine + GGGGGGGGGGL | - |
? | |
LGGGGGGGGGL + H2O | - |
Homo sapiens | L-leucine + GGGGGGGGGL | - |
? | |
LGGGL + H2O | - |
Homo sapiens | L-leucine + GGGL | - |
? | |
additional information | trimming of the N-terminal leucine residue by ERAP1 is faster for larger peptides of more than 10 residues long | Homo sapiens | ? | - |
? |