Cloned (Comment) | Organism |
---|---|
gene APE1, DNA and amino acid sequence determination and analysis, transcription is regulated by growth phase and by carbon source | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a mutant enzyme is defective in autophagocytosis and vacuole biogenesis | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | the zymogen remains in the cytoplasm and does not enter the secretory pathway | Saccharomyces cerevisiae | 5829 | - |
vacuole | the precursor enzyme is transported into the vacuole via the non-classic Cvt pathway by cytoplasmic double-membrane vesicles, which fuse with the vacuole releasing a single-membrane autophagic body into the vacuolar lumen where it is proteolytically maturated, overview | Saccharomyces cerevisiae | 5773 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cl- | specific, strong activation | Saccharomyces cerevisiae | |
Zn2+ | specific, strong activation, 2 mol per mol of enzyme | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
12 * 53000, active enzyme | Saccharomyces cerevisiae |
640000 | - |
- |
Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
gene APE1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | the zymogen is unglycosylated | Saccharomyces cerevisiae |
proteolytic modification | the inactive zymogen is activated by cleavage through cerevisin in a PEP4-dependent manner | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Leu-4-nitroanilide + H2O | - |
Saccharomyces cerevisiae | L-Leu + 4-nitroaniline | - |
? | |
additional information | the enzyme prefers substrates with N-terminal leucine or another hydrophobic amino acid residue, and is less active with Gly or charged amino acids at the P1 position | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | 12 * 53000, active enzyme | Saccharomyces cerevisiae |
More | dimeric and hexameric enzyme forms are inactive | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase I | - |
Saccharomyces cerevisiae |
Aminopeptidase III | - |
Saccharomyces cerevisiae |
aminopeptidase IV | - |
Saccharomyces cerevisiae |
Aminopeptidase yscI | - |
Saccharomyces cerevisiae |
LAP IV | - |
Saccharomyces cerevisiae |
More | the enzyme belongs to the peptidase family M18 | Saccharomyces cerevisiae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
4.7 |