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Literature summary for 3.4.11.21 extracted from
- Biochemical characterization of alpha-aspartyl dipeptidase. Cloning and expression of its gene (1998), Ann. N. Y. Acad. Sci., 864, 621-625.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | dipeptide sweeteners aspartame and alitame | Salmonella enterica subsp. enterica serovar Typhimurium |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyl diphosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium |  |
| Phenylglyoxal | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| pyridoxal 5'-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.94 | - |
Asp-Xaa | overexpressed enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
single polypeptide | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Asp-Xaa + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
Asp + Xaa | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| non-fusion protein expressed in Escherichia coli, 440fold to homogeneity | Salmonella enterica subsp. enterica serovar Typhimurium |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| solubilization of inclusion bodies by treatment with 0.6% Triton X-100, pH 10.0, 30 mM NaCl at 40°C for 20 min | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Asp-Xaa + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | Asp + Xaa | - |
r | |
| Asp-Xaa-Xaa + H2O | no activity on oligopeptides larger than tripeptides | Salmonella enterica subsp. enterica serovar Typhimurium | Asp + Xaa-Xaa | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| peptidase E | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
remarkably stable below 50°C, soon inactivated at 60°C | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | rather stable | Salmonella enterica subsp. enterica serovar Typhimurium |
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