Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bacitracin | activating at 0.2 mM | Oryctolagus cuniculus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | after preincubation total loss of activity | Oryctolagus cuniculus | |
dithiothreitol | 88% inhibition at 1 mM | Oryctolagus cuniculus | |
p-chloromercuribenzoate | - |
Oryctolagus cuniculus | |
Zn2+ | 50% inhibition at 0.4 M, after preincubation total inhibition | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | 2.8 | Asp-Xaa-Xaa-Xaa | - |
Oryctolagus cuniculus | |
0.25 | - |
angiotensin II | - |
Oryctolagus cuniculus | |
2 | - |
Asp-Ala-Pro-sulfamethoxazole | - |
Oryctolagus cuniculus | |
2 | - |
Asp-Ala-Pro-naphthylamide | - |
Oryctolagus cuniculus | |
2.3 | - |
Asp-Ala-Pro-sulfamethoxazole | overexpressed enzyme | Homo sapiens | |
8.4 | - |
Glu-Ala-Pro-naphthylamide | - |
Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Oryctolagus cuniculus | 5829 | - |
cytosol | - |
Rattus sp. | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS-PAGE | Oryctolagus cuniculus |
440000 | - |
native gel filtration | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin + H2O | Homo sapiens | intracellular protein and peptide metabolism | Asp + des-Asp-angiotensin | - |
? | |
angiotensin + H2O | Oryctolagus cuniculus | intracellular protein and peptide metabolism | Asp + des-Asp-angiotensin | - |
? | |
angiotensin + H2O | Rattus sp. | intracellular protein and peptide metabolism | Asp + des-Asp-angiotensin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Oryctolagus cuniculus | - |
rabbit | - |
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
722fold near homogeneity by conventional chromatography techniques | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Oryctolagus cuniculus | - |
brain | - |
Rattus sp. | - |
heart | - |
Rattus sp. | - |
intestine | - |
Rattus sp. | - |
kidney | - |
Rattus sp. | - |
liver | - |
Rattus sp. | - |
lung | - |
Rattus sp. | - |
spleen | - |
Rattus sp. | - |
testis | - |
Rattus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.013 | 0.058 | - |
Rattus sp. |
0.272 | 0.332 | overexpression in Escherichia coli | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin + H2O | intracellular protein and peptide metabolism | Homo sapiens | Asp + des-Asp-angiotensin | - |
? | |
angiotensin + H2O | intracellular protein and peptide metabolism | Oryctolagus cuniculus | Asp + des-Asp-angiotensin | - |
? | |
angiotensin + H2O | intracellular protein and peptide metabolism | Rattus sp. | Asp + des-Asp-angiotensin | - |
? | |
angiotensin II + H2O | octapeptides preferred to tetrapeptides | Oryctolagus cuniculus | Asp + angiotensin III | - |
? | |
Asp-Ala-Pro-2-naphthylamide + H2O | preferring acidic amino acids favouring aspartyl residues, requires a free alpha-amino group | Oryctolagus cuniculus | Asp + Ala-Pro-2-naphthylamide | - |
? | |
Asp-Ala-Pro-sulfamethoxazole + H2O | - |
Homo sapiens | Asp + Ala-Pro-sulfamethoxazole | - |
? | |
Asp-Ala-Pro-sulfamethoxazole + H2O | - |
Oryctolagus cuniculus | Asp + Ala-Pro-sulfamethoxazole | - |
? | |
Asp-Xaa-Xaa-Xaa + H2O | neutral or hydrophobic amino acids in P1 position preferred | Oryctolagus cuniculus | Asp + Xaa-Xaa-Xaa | - |
? | |
Glu-Ala-Pro-naphthylamide + H2O | - |
Oryctolagus cuniculus | Glu + Ala-Pro-naphthylamide | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Oryctolagus cuniculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
Zn2+ | after preincubation total inhibition | Oryctolagus cuniculus |