Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | treatment with 1,10-ortho-phenanthroline in order to deplete metal ions, results in a residual activity of about 8% compared to the initial activity. Activity is partially restored by the addition of divalent metal cations with Zn2+ being the most potent | Eimeria tenella |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0165 | - |
norleucyl-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella | |
0.0236 | - |
(S)-2-amino-4-cyclohexylbutanoyl-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella | |
0.0262 | - |
homophenylalanyl-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella | |
0.0277 | - |
homoargininyl-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella | |
0.0302 | - |
styryl-Ala-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella | |
0.0314 | - |
L-methionyl-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella | |
0.0354 | - |
norvalinyl-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella | |
0.0598 | - |
Ala-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | treatment with 1,10-phenanthroline in order to deplete metal ions, results in a residual activity of about 8% compared to the initial activity. Activity is partially restored by the addition of divalent metal cations with Zn2+ being the most potent | Eimeria tenella |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eimeria tenella | V5T9T8 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | isoform APN1 is synthesized as a 120-kDa precursor and cleaved into 96-, 68-, and 38-kDa forms during sporulation | Eimeria tenella |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-amino-4-cyclohexylbutanoyl-7-amido-4-methylcoumarin + H2O | - |
Eimeria tenella | (S)-2-amino-4-cyclohexylbutanoic acid + 7-amino-4-methylcoumarin | - |
? | |
Ala-7-amido-4-methylcoumarin + H2O | - |
Eimeria tenella | alanine + 7-amino-4-methylcoumarin | - |
? | |
homoargininyl-7-amido-4-methylcoumarin + H2O | - |
Eimeria tenella | homoarginine + 7-amino-4-methylcoumarin | - |
? | |
homophenylalanyl-7-amido-4-methylcoumarin + H2O | - |
Eimeria tenella | homophenylalanine + 7-amino-4-methylcoumarin | - |
? | |
L-methionyl-7-amido-4-methylcoumarin + H2O | - |
Eimeria tenella | L-methionine + 7-amino-4-methylcoumarin | - |
? | |
norleucyl-7-amido-4-methylcoumarin + H2O | - |
Eimeria tenella | norleucine + 7-amino-4-methylcoumarin | - |
? | |
norvalinyl-7-amido-4-methylcoumarin + H2O | - |
Eimeria tenella | norvaline + 7-amino-4-methylcoumarin | - |
? | |
styryl-Ala-7-amido-4-methylcoumarin + H2O | - |
Eimeria tenella | styryl-alanine + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APN1 | - |
Eimeria tenella |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.456 | - |
Ala-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella | |
0.575 | - |
homoargininyl-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella | |
0.577 | - |
styryl-Ala-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella | |
0.658 | - |
norvalinyl-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella | |
0.704 | - |
L-methionyl-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella | |
0.71 | - |
norleucyl-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella | |
0.737 | - |
(S)-2-amino-4-cyclohexylbutanoyl-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella | |
0.836 | - |
homophenylalanyl-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella |
Organism | Comment | Expression |
---|---|---|
Eimeria tenella | isoform APN1 is present during the intracellular life cycle stages in both the parasite cytoplasm and the parasite nucleus | additional information |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.6 | - |
Ala-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella | |
18.6 | - |
norvalinyl-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella | |
19.1 | - |
styryl-Ala-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella | |
20.8 | - |
homoargininyl-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella | |
22.4 | - |
L-methionyl-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella | |
31.9 | - |
homophenylalanyl-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella | |
43 | - |
norleucyl-7-amido-4-methylcoumarin | pH 7.4, 37°C | Eimeria tenella |