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Literature summary for 3.4.11.19 extracted from

  • Marui, J.; Matsushita-Morita, M.; Tada, S.; Hattori, R.; Suzuki, S.; Amano, H.; Ishida, H.; Yamagata, Y.; Takeuchi, M.; Kusumoto, K.
    Enzymatic properties of the glycine D-alanine aminopeptidase of Aspergillus oryzae and its activity profiles in liquid-cultured mycelia and solid-state rice culture (rice koji) (2012), Appl. Microbiol. Biotechnol., 93, 655-669.
    View publication on PubMed
Search for more literature for 3.4.11.19

Cloned(Commentary)

Cloned (Comment) Organism
expression in Aspergillus oryzae Aspergillus oryzae

Inhibitors

Inhibitors Comment Organism Structure
AEBSF 1 mM, 45% residual activity Aspergillus oryzae
Cd2+ 1 mM, less than 5% residual activity Aspergillus oryzae
Co2+ 1 mM, 50% residual activity Aspergillus oryzae
Cu2+ 1 mM, 5% residual activity Aspergillus oryzae
EDTA 1 mM, 40% residual activity Aspergillus oryzae
Ni2+ 1 mM, 20% residual activity Aspergillus oryzae
Zn2+ 1 mM, less than 5% residual activity Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 Gly 4-nitroanilide pH 9.0, 30°C Aspergillus oryzae
0.33
-
 D-Ala 4-nitroanilide pH 9.0, 30°C Aspergillus oryzae
6.7
-
 L-Ala 4-nitroanilide pH 9.0, 30°C Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59300
-
 2 * 59300, calculated Aspergillus oryzae
126300
-
 gel filtration Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae G7H7Y1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ala 4-nitroanilide + H2O 93% of the activity with Gly 4-nitroanilide Aspergillus oryzae D-Ala + 4-nitroaniline
-
 ?
Gly 4-nitroanilide + H2O
-
 Aspergillus oryzae Gly + 4-nitroaniline
-
 ?
Gly-L-Ala + H2O
-
 Aspergillus oryzae Gly + L-Ala
-
 ?
Gly-L-Cys + H2O
-
 Aspergillus oryzae Gly + L-Cys
-
 ?
L-Ala 4-nitroanilide + H2O 12% of the activity with Gly 4-nitroanilide Aspergillus oryzae L-Ala + 4-nitroaniline
-
 ?
L-Ala-D-Ala + H2O
-
 Aspergillus oryzae L-Ala + D-Ala
-
 ?
additional information enzyme shows strict D-stereospecific aminopeptidase activity. No or very poor activity with beta-Ala 4-nitroanilide, L-Leu 4-nitroanilide, D-Leu 4-nitroanilide,L-Met 4-nitroanilide, L-Pro 4-nitroanilide, L-Val 4-nitroanilide, L-Ile 4-nitroanilide, L-Phe 4-nitroanilide, D-Phe 4-nitroanilide, L-Lys 4-nitroanilide, L-Arg 4-nitroanilide, L-His 4-nitroanilide, L-Asp 4-nitroanilide, L-Glu 4-nitroanilide. No substrates: L-Ala-Gly and L-Cys-Gly dipeptides and L-Cys-Gly-Gly tripeptide Aspergillus oryzae ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 59300, calculated Aspergillus oryzae

Synonyms

Synonyms Comment Organism
GdaA
-
 Aspergillus oryzae
glycine D-alanine aminopeptidase
-
 Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Aspergillus oryzae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2100
-
 L-Ala 4-nitroanilide pH 9.0, 30°C Aspergillus oryzae
2550
-
 D-Ala 4-nitroanilide pH 9.0, 30°C Aspergillus oryzae
2783
-
 Gly 4-nitroanilide pH 9.0, 30°C Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 10
-
 Aspergillus oryzae

pH Range

pH Minimum pH Maximum Comment Organism
5 11
-
 Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 11
-
 Aspergillus oryzae

General Information

General Information Comment Organism
physiological function no significant differences are observed in the vegetative growth and conidiation on minimal and complete media in the enzyme gene disruptant or overexpression strains Aspergillus oryzae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
313
-
 L-Ala 4-nitroanilide pH 9.0, 30°C Aspergillus oryzae
7750
-
 D-Ala 4-nitroanilide pH 9.0, 30°C Aspergillus oryzae
11000
-
 Gly 4-nitroanilide pH 9.0, 30°C Aspergillus oryzae