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Literature summary for 3.4.11.19 extracted from

  • Delmarcelle, M.; Boursoit, M.C.; Filee, P.; Baurin, S.L.; Frere, J.M.; Joris, B.
    Specificity inversion of Ochrobactrum anthropi D-aminopeptidase to a D,D-carboxypeptidase with new penicillin binding activity by directed mutagenesis (2005), Protein Sci., 14, 2296-2303.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.19

Protein Variants

Protein Variants Comment Organism
additional information enzyme consists of N-terminal domain DAP-A and C-terminal domains DAP-B and DAB-C. Coexpression of DAP-A plus DAP-BC results in a complex that copurifies on Ni-NTA-column and shows a 10fold increase in KM-value compared to wild-type. Mutant lacking gamma-loop residues 476-486, no enzymic activity but small D,D-carboxypeptidase and beta-lactamase activity. Mutant lacking gamma-loop residues 476-486 plus mutation N275R, no enzymic activity but small D,D-carboxypeptidase and beta-lactamase activity. Brucella anthropi

Inhibitors

Inhibitors Comment Organism Structure
nitrocefin inhibitory on D,D-carboxypeptidase activity of mutant lacking residues 476-486 plus mutation N275R Brucella anthropi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.46
-
 D-Ala-4-nitroanilide wild-type, pH 8.0, 30°C Brucella anthropi
0.51
-
 D-Ala-4-nitroanilide mutant lacking residues 476-486 plus domains DAP-BC, pH 8.0, 30°C Brucella anthropi
5.1
-
 D-Ala-4-nitroanilide complex of domain DAP-A plus domains DAP-BC, pH 8.0, 30°C Brucella anthropi
26
-
 nitrocefin mutant lacking residues 476-486 plus mutation N275R, pH 7.0, 30°C Brucella anthropi
29
-
 nitrocefin mutant lacking residues 476-486, pH 7.0, 30°C Brucella anthropi
49
-
 Nalpha,Nalpha-Ac2-L-Lys-D-Ala-D-Ala mutant lacking residues 476-486 plus mutation N275R, pH 8.0, 37°C Brucella anthropi
185
-
 Nalpha-Ac-L-Lys-D-Ala-D-Ala mutant lacking residues 476-486 plus mutation N275R, pH 8.0, 37°C Brucella anthropi

Organism

Organism UniProt Comment Textmining
Brucella anthropi
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ala-4-nitroanilide + H2O
-
 Brucella anthropi D-Ala + 4-nitroaniline
-
 ?
fluoresceyl-Gly-6-aminopenicillanic acid + H2O penicillin-binding activity of mutant lacking residues 476-486 Brucella anthropi ?
-
 ?
Nalpha, Nalpha-Ac2-L-Lys-D-Ala-D-Ala + H2O wild-type, no substrate. Mutant lacking residues 476-486 with or without mutation N275R, small D,D-carboxypeptidase activity Brucella anthropi Nalpha, Nalpha-Ac2-L-Lys-D-Ala + D-Ala
-
 ?
Nalpha-Ac-L-Lys-D-Ala-D-Ala + H2O wild-type and mutant lacking residues 476-486, no substrate. Mutant lacking residues 476-486 plus mutation N275R, small D,D-carboxypeptidase activity Brucella anthropi Nalpha-Ac-L-Lys-D-Ala + D-Ala
-
 ?
nitrocefin + H2O
-
 Brucella anthropi ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0008
-
 nitrocefin mutant lacking residues 476-486 plus mutation N275R, pH 8.0, 30°C Brucella anthropi
0.0016
-
 nitrocefin mutant lacking residues 476-486, pH 8.0, 30°C Brucella anthropi
0.2
-
 Nalpha-Ac-L-Lys-D-Ala-D-Ala mutant lacking residues 476-486 plus mutation N275R, pH 8.0, 37°C Brucella anthropi
0.62
-
 Nalpha, Nalpha-Ac2-L-Lys-D-Ala-D-Ala mutant lacking residues 476-486 plus mutation N275R, pH 8.0, 37°C Brucella anthropi
33
-
 D-Ala-4-nitroanilide mutant lacking residues 476-486 plus domains DAP-BC, pH 8.0, 30°C Brucella anthropi
210
-
 D-Ala-4-nitroanilide complex of domain DAP-A plus domains DAP-BC, pH 8.0, 30°C Brucella anthropi
580
-
 D-Ala-4-nitroanilide wild-type, pH 8.0, 30°C Brucella anthropi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.009
-
 nitrocefin pH 7.0, 30°C Brucella anthropi