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Literature summary for 3.4.11.19 extracted from

  • Asano, Y.; Nakazawa, A.; Kato, Y.; Kondo, K.
    Properties of a novel D-stereospecific aminopeptidase from Ochrobactrum anthropi (1989), J. Biol. Chem., 264, 14233-14239.
    View publication on PubMed
Search for more literature for 3.4.11.19

Activating Compound

Activating Compound Comment Organism Structure
additional information thiol-dependent Brucella anthropi

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid)
-
 Brucella anthropi
Ag+
-
 Brucella anthropi
ampicillin
-
 Brucella anthropi
Ca2+ 10 mM Brucella anthropi
Cd2+ 10 mM Brucella anthropi
Cu2+ 10 mM Brucella anthropi
Hg2+
-
 Brucella anthropi
hydroxylamine
-
 Brucella anthropi
additional information probably has serine at the active centre, not: EDTA (0.2 mM) Brucella anthropi
NEM
-
 Brucella anthropi
Ni2+ 10 mM Brucella anthropi
PCMB
-
 Brucella anthropi
Zn2+ 10 mM Brucella anthropi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.32
-
 D-Ala4
-
 Brucella anthropi
0.37
-
 D-Ala-Gly-Gly
-
 Brucella anthropi
0.51
-
 D-alanine-4-nitroanilide
-
 Brucella anthropi
0.51
-
 D-Ala benzylamide
-
 Brucella anthropi
0.57
-
 D-Ala-D-Ala-D-Ala
-
 Brucella anthropi
0.65
-
 D-alanine amide
-
 Brucella anthropi
0.65
-
 D-Ala-L-Ala-L-Ala
-
 Brucella anthropi
0.73
-
 D-Ala-n-butylamide
-
 Brucella anthropi
0.98
-
 D-Ala-Gly
-
 Brucella anthropi
1.03
-
 D-Ala-L-Ala
-
 Brucella anthropi
2.27
-
 D-Ala-3-aminopentane amide
-
 Brucella anthropi
10.2
-
 D-Ala-D-Ala
-
 Brucella anthropi
18.3
-
 D-2-Aminobutyric acid amide
-
 Brucella anthropi
22.3
-
 glycine amide
-
 Brucella anthropi
27
-
 D-Serine amide
-
 Brucella anthropi
100
-
 D-threonine amide
-
 Brucella anthropi

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not metal-dependent Brucella anthropi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 2 * 59000, SDS-PAGE Brucella anthropi
122000
-
 gel filtration Brucella anthropi

Organism

Organism UniProt Comment Textmining
Brucella anthropi
-
 SCRC C1-38
-
Brucella anthropi SCRC C1-38
-
 SCRC C1-38
-

Purification (Commentary)

Purification (Comment) Organism
-
 Brucella anthropi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Brucella anthropi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-2-aminobutyric acid amide + H2O
-
 Brucella anthropi D-aminobutyric acid + NH3
-
 ?
D-2-aminobutyric acid amide + H2O
-
 Brucella anthropi SCRC C1-38 D-aminobutyric acid + NH3
-
 ?
D-Ala 4-nitroanilide + H2O
-
 Brucella anthropi D-Ala + 4-nitroaniline
-
 ?
D-Ala-3-aminopentane amide + H2O
-
 Brucella anthropi ?
-
 ?
D-Ala-D-Ala + H2O
-
 Brucella anthropi D-Ala
-
 ?
D-Ala-Gly + H2O
-
 Brucella anthropi D-Ala + Gly
-
 ?
D-Ala-Gly + H2O
-
 Brucella anthropi SCRC C1-38 D-Ala + Gly
-
 ?
D-Ala-Gly-Gly + H2O
-
 Brucella anthropi D-Ala + Gly-Gly
-
 ?
D-Ala-L-Ala-L-Ala + H2O
-
 Brucella anthropi D-Ala + Ala-Ala
-
 ?
D-Ala4 + H2O
-
 Brucella anthropi ?
-
 ?
D-alanine amide + H2O
-
 Brucella anthropi D-Ala + NH3
-
 ?
D-alanine amide + H2O
-
 Brucella anthropi SCRC C1-38 D-Ala + NH3
-
 ?
D-alanine benzylamide + H2O
-
 Brucella anthropi D-Ala + benzylamine
-
 ?
D-alanine n-butylamide + H2O
-
 Brucella anthropi D-Ala + n-butylamine
-
 ?
D-serine amide + H2O
-
 Brucella anthropi D-serine + NH3
-
 ?
D-serine amide + H2O
-
 Brucella anthropi SCRC C1-38 D-serine + NH3
-
 ?
D-threonine amide + H2O
-
 Brucella anthropi D-Thr + NH3
-
 ?
glycine amide + H2O
-
 Brucella anthropi Gly + NH3
-
 ?
additional information strict D-stereospecificity, the enzyme prefers peptides to amino acid arylamides or amino acid amides Brucella anthropi ?
-
 ?
additional information strict D-stereospecificity, the enzyme prefers peptides to amino acid arylamides or amino acid amides Brucella anthropi SCRC C1-38 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 59000, SDS-PAGE Brucella anthropi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Brucella anthropi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 pH 7.0-10.9, 1 h, no loss of activity Brucella anthropi
45
-
 pH 8.0, 10 min, about 80% of original activity retained Brucella anthropi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Brucella anthropi

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10 30°C, 1 h, no loss of activity Brucella anthropi
8
-
 45°C, 10 min, about 80% of the original activity retained Brucella anthropi

pI Value

Organism Comment pI Value Maximum pI Value
Brucella anthropi
-
-
 4.2