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Literature summary for 3.4.11.18 extracted from

  • Munkhjargal, T.; Ishizaki, T.; Guswanto, A.; Takemae, H.; Yokoyama, N.; Igarashi, I.
    Molecular and biochemical characterization of methionine aminopeptidase of Babesia bovis as a potent drug target (2016), Vet. Parasitol., 221, 14-23 .
    View publication on PubMed
Search for more literature for 3.4.11.18

Application

Application Comment Organism
medicine recombinant MetAP1 is able to provide considerable protection to mice against a Babesia microti challenge infection based on a reduction in peak parasitemia levels and earlier clearance of the parasite as compared with control mice Babesia bovis

Inhibitors

Inhibitors Comment Organism Structure
amastatin 1 mM, 37.6% of initial activity Babesia bovis
bestatin 1 mM, 35.9% of initial activity Babesia bovis
E-64 1 mM, 89.1% of initial activity Babesia bovis
EDTA 1 mM, 37.8% of initial activity Babesia bovis
PMSF 1 mM, 82.8% of initial activity Babesia bovis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0143
-
 L-methionine 4-methylcoumaryl-7-amide pH 7.5, temperature not specified in the publication Babesia bovis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm cytoplasm of merozoite Babesia bovis 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.1 mM, 120% of initial activity Babesia bovis
Mg2+ 0.1 mM, 146% of initial activity Babesia bovis
Mn2+ 0.1 mM, 344% of initial activity Babesia bovis

Organism

Organism UniProt Comment Textmining
Babesia bovis S6BKQ9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine 4-methylcoumaryl-7-amide + H2O
-
 Babesia bovis L-methionine + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 40867, calculated from sequence, x * 66800, recombinant GST fusion protein Babesia bovis

Synonyms

Synonyms Comment Organism
MetAP1
-
 Babesia bovis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Babesia bovis

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5
-
 Babesia bovis

pI Value

Organism Comment pI Value Maximum pI Value
Babesia bovis
-
-
 7

General Information

General Information Comment Organism
physiological function recombinant enzyme is immunogenic, eliciting a high antibody titer in mice. Recombinant MetAP1 stimulates the production of IFN-gamma and IL-12 but not IL-4 in the host Babesia bovis