Literature summary for 3.4.11.18 extracted from

Arya, T.; Kishor, C.; Saddanapu, V.; Reddi, R.; Addlagatta, A.
Discovery of a new genetic variant of methionine aminopeptidase from Streptococci with possible post-translational modifications: biochemical and structural characterization (2013), PLoS ONE, 8, e75207.

Search for more literature for 3.4.11.18

Crystallization (Commentary)

Crystallization (Comment)	Organism
contains two molecules in the asymmetric unit and well ordered inserts with structural features that corroborate the possible posttranslational modification. Both the new inserts present structurally align with the P-X-X-P motif found in other type I Metionine aminopeptidases as well as the 60 amino acid insert in the human type II. In addition, one of the beta-hairpins within in the catalytic domain undergoes a flip placing a residue which is essential for enzyme activity away from the active site and the beta-hairpin loop of this secondary structure in the active site obstructing substrate binding	Streptococcus pneumoniae

Crystallization (Comment)

Organism

contains two molecules in the asymmetric unit and well ordered inserts with structural features that corroborate the possible posttranslational modification. Both the new inserts present structurally align with the P-X-X-P motif found in other type I Metionine aminopeptidases as well as the 60 amino acid insert in the human type II. In addition, one of the beta-hairpins within in the catalytic domain undergoes a flip placing a residue which is essential for enzyme activity away from the active site and the beta-hairpin loop of this secondary structure in the active site obstructing substrate binding

Streptococcus pneumoniae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.513	-	L-Met 4-nitroanilide	pH 7.5, 30°C	Streptococcus pneumoniae

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	B2IQ22	-	-
Streptococcus pneumoniae CGSP14	B2IQ22	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
L-Met 4-nitroanilide + H2O	-	Streptococcus pneumoniae	L-Met + 4-nitroaniline	-	?
L-Met 4-nitroanilide + H2O	-	Streptococcus pneumoniae CGSP14	L-Met + 4-nitroaniline	-	?
additional information	no substrates: 4-nitroanilides of Val, Arg, Leu, Glu, Gly, Lys, Phe, Pro,Tyr, Ile	Streptococcus pneumoniae	?	-	?
additional information	no substrates: 4-nitroanilides of Val, Arg, Leu, Glu, Gly, Lys, Phe, Pro,Tyr, Ile	Streptococcus pneumoniae CGSP14	?	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0055	-	L-Met 4-nitroanilide	pH 7.5, 30°C	Streptococcus pneumoniae

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.0108	-	L-Met 4-nitroanilide	pH 7.5, 30°C	Streptococcus pneumoniae