Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
C105S | about 50% loss of activiy | Mycobacterium tuberculosis |
D142A | almost complete loss of activity | Mycobacterium tuberculosis |
T94C | slight enhancement of activity | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
actinonin | 0.01 mM, 40% loss of activity; 0.01 mM, 70% loss of activity | Mycobacterium tuberculosis | |
bestatin | 0.01 mM, 25% loss of activity; 0.01 mM, 50% loss of activity | Mycobacterium tuberculosis | |
methionine | feedback inhibition. 70% inhibition at 0.1 mM | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.154 | - |
L-Met 4-nitroanilide | presence of Ni2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
0.193 | - |
L-Met 4-nitroanilide | presence of Co2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
0.466 | - |
L-Met 4-nitroanilide | presence of Ni2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
0.52 | - |
L-Met 4-nitroanilide | presence of Co2+, pH 8.0, 22°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | and Ni2+, best activators. Highest activity at 0.1 mM | Mycobacterium tuberculosis | |
Co2+ | may substitute for Ni2+. Highest activity at 0.04 mM | Mycobacterium tuberculosis | |
Mn2+ | may substitute for Co2+ or Ni2+, highest activity at 0.4 mM | Mycobacterium tuberculosis | |
Ni2+ | and Co2+, best activators. Highest activity at 0.1 mM | Mycobacterium tuberculosis | |
Ni2+ | best activator. Highest activity at 0.1 mM | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, SDS-PAGE, recombinant protein | Mycobacterium tuberculosis |
37000 | - |
x * 37000, SDS-PAGE, recombinant protein | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WK19 | - |
- |
Mycobacterium tuberculosis | P9WK21 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WK19 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WK21 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Met 4-nitroanilide + H2O | - |
Mycobacterium tuberculosis | L-Met + 4-nitroaniline | - |
? | |
L-Met 4-nitroanilide + H2O | - |
Mycobacterium tuberculosis H37Rv | L-Met + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, SDS-PAGE, recombinant protein | Mycobacterium tuberculosis |
? | x * 37000, SDS-PAGE, recombinant protein | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
MAP-2 | - |
Mycobacterium tuberculosis |
MAP11 | - |
Mycobacterium tuberculosis |
mAPA | - |
Mycobacterium tuberculosis |
MapB | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Mycobacterium tuberculosis |
55 | - |
- |
Mycobacterium tuberculosis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
no activity above | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.078 | - |
L-Met 4-nitroanilide | presence of Co2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
0.08 | - |
L-Met 4-nitroanilide | presence of Co2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
0.08 | - |
L-Met 4-nitroanilide | presence of Ni2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
0.133 | - |
L-Met 4-nitroanilide | presence of Ni2+, pH 8.0, 22°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
153 | - |
L-Met 4-nitroanilide | presence of Co2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
171 | - |
L-Met 4-nitroanilide | presence of Ni2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
404 | - |
L-Met 4-nitroanilide | presence of Co2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
867 | - |
L-Met 4-nitroanilide | presence of Ni2+, pH 8.0, 22°C | Mycobacterium tuberculosis |