Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-(2-chlorophenyl)-2-furohydrazide | - |
Escherichia coli | |
EDTA | - |
Escherichia coli | |
EGTA | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
Met-Gly-Met-Met | Mn2+-activated enzyme, pH 7.5, 21°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activation constant 0.003 mM | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli K-12 MG1655 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ion exchange chromatography (Q-Sepharose), hydrophobic interaction chromatography (Phenyl Sepharose), gel filtration (Sephacryl S-200) | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Met-Gly-Met-Met + H2O | - |
Escherichia coli | L-methionine + Gly-Met-Met | - |
? | |
Met-Gly-Met-Met + H2O | - |
Escherichia coli K-12 MG1655 | L-methionine + Gly-Met-Met | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methionine aminopeptidase | - |
Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.002 | - |
Mn2+-activated enzyme (0.004 mM MnCl2), pH 7.5, 21°C | Escherichia coli | 5-(2-chlorophenyl)-2-furohydrazide |