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Literature summary for 3.4.11.18 extracted from

  • Shapiro, A.B.; Gao, N.; Thresher, J.; Walkup, G.K.; Whiteaker, J.
    A high-throughput absorbance-based assay for methionine produced by methionine aminopeptidase using S-adenosyl-L-methionine synthetase (2011), J. Biomol. Screen., 16, 494-505.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
5-(2-chlorophenyl)-2-furohydrazide
-
Escherichia coli
EDTA
-
Escherichia coli
EGTA
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
Met-Gly-Met-Met Mn2+-activated enzyme, pH 7.5, 21°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activation constant 0.003 mM Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli K-12 MG1655
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ion exchange chromatography (Q-Sepharose), hydrophobic interaction chromatography (Phenyl Sepharose), gel filtration (Sephacryl S-200) Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Met-Gly-Met-Met + H2O
-
Escherichia coli L-methionine + Gly-Met-Met
-
?
Met-Gly-Met-Met + H2O
-
Escherichia coli K-12 MG1655 L-methionine + Gly-Met-Met
-
?

Synonyms

Synonyms Comment Organism
methionine aminopeptidase
-
Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.002
-
Mn2+-activated enzyme (0.004 mM MnCl2), pH 7.5, 21°C Escherichia coli 5-(2-chlorophenyl)-2-furohydrazide