Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
D117A | lining the perimeter of the active site, no activity | Mycobacterium tuberculosis |
D131A | no activity | Mycobacterium tuberculosis |
DELTA 2-10 | 30% activity with 10fold amount of enzyme in the assay relative to wild type | Mycobacterium tuberculosis |
DELTA 2-15 | 30% activity with 10fold amount of enzyme in the assay relative to wild type | Mycobacterium tuberculosis |
DELTA 2-20 | no activity | Mycobacterium tuberculosis |
DELTA 2-30 | no activity | Mycobacterium tuberculosis |
DELTA 2-40 | no activity | Mycobacterium tuberculosis |
E219V | predicted active site residue, no activity | Mycobacterium tuberculosis |
E238A | predicted active site residue, no activity | Mycobacterium tuberculosis |
H114A | predicted active site residue, no activity | Mycobacterium tuberculosis |
H193A | predicted active site residue, no activity | Mycobacterium tuberculosis |
H212A | predicted active site residue, no activity | Mycobacterium tuberculosis |
H88A | predicted active site residue, no activity | Mycobacterium tuberculosis |
P19A | 65% activity relative to wild type | Mycobacterium tuberculosis |
P19G | 35% activity relative to wild type | Mycobacterium tuberculosis |
V18A | 30% activity relative to wild type | Mycobacterium tuberculosis |
V18A/P19A | no activity | Mycobacterium tuberculosis |
V18G | no activity | Mycobacterium tuberculosis |
V18G/P19G | no activity | Mycobacterium tuberculosis |
W236L | no activity | Mycobacterium tuberculosis |
W255L | no activity | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
amastatin | - |
Mycobacterium tuberculosis | |
EDTA | - |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.36 | - |
Met-Ala-Ser | pH 7.5, 30°C | Mycobacterium tuberculosis | |
2.85 | - |
Met-Gly-Met-Met | pH 7.5, 30°C | Mycobacterium tuberculosis | |
3.3 | - |
Met-Ala-Ser | pH 7.5, 30°C | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37300 | - |
x * 37300, SDS-PAGE | Mycobacterium tuberculosis |
39100 | - |
x * 39100, SDS-PAGE | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Met-Ala-Ser + H2O | - |
Mycobacterium tuberculosis | L-methionine + Ala-Ser | - |
? | |
Met-Gly-Met-Met + H2O | - |
Mycobacterium tuberculosis | L-methionine + Gly-Met-Met | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37300, SDS-PAGE | Mycobacterium tuberculosis |
? | x * 39100, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
methionine aminopeptidase type 1a | - |
Mycobacterium tuberculosis |
methionine aminopeptidase type 1c | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 37 | - |
Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
85% activity after 10 min at 50°C, Tm: 53,6°C (determination by CD spectroscopy) | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.63 | - |
Met-Gly-Met-Met | calculated with 29.5 kDa as molecular mass, pH 7.5, 30°C | Mycobacterium tuberculosis | |
11.7 | - |
Met-Ala-Ser | calculated with 29.5 kDa as molecular mass, pH 7.5, 30°C | Mycobacterium tuberculosis | |
1516 | - |
Met-Ala-Ser | calculated with 33.1 kDa as molecular mass, pH 7.5, 30°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.32 | - |
Met-Ala-Ser | pH 7.5, 30°C | Mycobacterium tuberculosis | |
3.47 | - |
Met-Gly-Met-Met | pH 7.5, 30°C | Mycobacterium tuberculosis | |
1156 | - |
Met-Ala-Ser | pH 7.5, 30°C | Mycobacterium tuberculosis |