Cloned (Comment) | Organism |
---|---|
His-tagged protein expressed in Escherichia coli BL21(DE3) | Acinetobacter baumannii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(2-methyl-1,3-thiazol-4-yl)benzene-1,2-diol | - |
Acinetobacter baumannii | |
4-(3-methylthiophen-2-yl)benzene-1,2-diol | - |
Acinetobacter baumannii | |
5-(2,5-dichlorophenyl)furan-2-carboxylic acid | - |
Acinetobacter baumannii | |
5-(2-chlorophenyl)furan-2-carboxylic acid | - |
Acinetobacter baumannii | |
5-[(2,4-dichlorobenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | - |
Acinetobacter baumannii | |
5-[(4-fluorobenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | - |
Acinetobacter baumannii | |
5-[(4-methylbenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | - |
Acinetobacter baumannii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.111 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.005 mM Ni2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.115 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.020 mM Mn2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.13 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.020 mM Co2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.144 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.020 mM Ni2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.154 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.020 mM Mn2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.155 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.0025 mM Fe2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.161 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.0025 mM Fe2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.168 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.020 mM Co2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | activator after removal of divalent metal ions with Chelex-100 | Acinetobacter baumannii | |
Fe2+ | activator after removal of divalent metal ions with EDTA | Acinetobacter baumannii | |
Ni2+ | activator after removal of divalent metal ions with Chelex-100 | Acinetobacter baumannii | |
Ni2+ | activator after removal of divalent metal ions with EDTA, less efficient than Fe2+ | Acinetobacter baumannii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baumannii | A0A1E3MBK2 | - |
- |
Acinetobacter baumannii ATCC 17978 | A0A1E3MBK2 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Acinetobacter baumannii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Met-4-methylcoumaryl-7-amide + H2O | - |
Acinetobacter baumannii | Met + 7-amino-4-methylcoumarin | - |
? | |
Met-4-methylcoumaryl-7-amide + H2O | - |
Acinetobacter baumannii ATCC 17978 | Met + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methionine aminopeptidase AbMetAPx | - |
Acinetobacter baumannii |
methionine aminopeptidase AbMetAPy | - |
Acinetobacter baumannii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000508 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.020 mM Mn2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.000055 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.020 mM Mn2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.000137 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.020 mM Co2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.000159 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.020 mM Co2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.000263 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.0025 mM Fe2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.000305 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.005 mM Ni2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.000312 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.020 mM Ni2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.000449 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.0025 mM Fe2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00038 | - |
after removal of divalent metal ions activated with 0.02 mM Co2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-[(2,4-dichlorobenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | |
0.0004 | - |
after removal of divalent metal ions activated with 0.02 mM Ni2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-[(2,4-dichlorobenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | |
0.00051 | - |
after removal of divalent metal ions activated with 0.0025 mM Fe2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 4-(3-methylthiophen-2-yl)benzene-1,2-diol | |
0.00064 | - |
after removal of divalent metal ions activated with 0.02 mM Co2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-[(4-fluorobenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | |
0.00065 | - |
after removal of divalent metal ions activated with 0.0025 mM Fe2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 4-(3-methylthiophen-2-yl)benzene-1,2-diol | |
0.00078 | - |
after removal of divalent metal ions activated with 0.02 mM Co2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-[(2,4-dichlorobenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | |
0.00088 | - |
after removal of divalent metal ions activated with 0.02 mM Co2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-[(4-fluorobenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | |
0.00092 | - |
after removal of divalent metal ions activated with 0.02 mM Mn2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-(2-chlorophenyl)furan-2-carboxylic acid | |
0.00095 | - |
after removal of divalent metal ions activated with 0.0025 mM Fe2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 4-(2-methyl-1,3-thiazol-4-yl)benzene-1,2-diol | |
0.0021 | - |
after removal of divalent metal ions activated with 0.02 mM Co2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-[(4-methylbenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | |
0.0022 | - |
after removal of divalent metal ions activated with 0.02 mM Ni2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-[(4-fluorobenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | |
0.0038 | - |
after removal of divalent metal ions activated with 0.02 mM Co2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-[(4-methylbenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | |
0.0044 | - |
after removal of divalent metal ions activated with 0.02 mM Ni2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-[(4-methylbenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | |
0.0049 | - |
after removal of divalent metal ions activated with 0.005 mM Ni2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-[(2,4-dichlorobenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | |
0.0061 | - |
after removal of divalent metal ions activated with 0.02 mM Mn2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-(2,5-dichlorophenyl)furan-2-carboxylic acid | |
0.0071 | - |
after removal of divalent metal ions activated with 0.02 mM Mn2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-(2,5-dichlorophenyl)furan-2-carboxylic acid | |
0.013 | - |
after removal of divalent metal ions activated with 0.02 mM Mn2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-(2-chlorophenyl)furan-2-carboxylic acid | |
0.014 | - |
after removal of divalent metal ions activated with 0.02 mM Mn2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-[(2,4-dichlorobenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | |
0.021 | - |
after removal of divalent metal ions activated with 0.02 mM Co2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 4-(3-methylthiophen-2-yl)benzene-1,2-diol | |
0.022 | - |
after removal of divalent metal ions activated with 0.02 mM Mn2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 4-(3-methylthiophen-2-yl)benzene-1,2-diol | |
0.023 | - |
after removal of divalent metal ions activated with 0.005 mM Ni2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-[(4-fluorobenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | |
0.024 | - |
after removal of divalent metal ions activated with 0.02 mM Co2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 4-(2-methyl-1,3-thiazol-4-yl)benzene-1,2-diol | |
0.027 | - |
after removal of divalent metal ions activated with 0.005 mM Ni2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 4-(3-methylthiophen-2-yl)benzene-1,2-diol | |
0.03 | - |
after removal of divalent metal ions activated with 0.02 mM Mn2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 4-(3-methylthiophen-2-yl)benzene-1,2-diol | |
0.032 | - |
after removal of divalent metal ions activated with 0.0025 mM Fe2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 4-(2-methyl-1,3-thiazol-4-yl)benzene-1,2-diol | |
0.033 | - |
after removal of divalent metal ions activated with 0.02 mM Co2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 4-(3-methylthiophen-2-yl)benzene-1,2-diol | |
0.034 | - |
after removal of divalent metal ions activated with 0.02 mM Mn2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 4-(2-methyl-1,3-thiazol-4-yl)benzene-1,2-diol | |
0.034 | - |
after removal of divalent metal ions activated with 0.02 mM Mn2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-[(4-fluorobenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | |
0.039 | - |
after removal of divalent metal ions activated with 0.02 mM Co2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 4-(2-methyl-1,3-thiazol-4-yl)benzene-1,2-diol | |
0.044 | - |
after removal of divalent metal ions activated with 0.005 mM Ni2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-[(4-methylbenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | |
0.055 | - |
after removal of divalent metal ions activated with 0.02 mM Ni2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 4-(3-methylthiophen-2-yl)benzene-1,2-diol | |
0.056 | - |
after removal of divalent metal ions activated with 0.02 mM Ni2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 4-(2-methyl-1,3-thiazol-4-yl)benzene-1,2-diol | |
0.064 | - |
after removal of divalent metal ions activated with 0.02 mM Mn2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-[(2,4-dichlorobenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | |
0.072 | - |
after removal of divalent metal ions activated with 0.005 mM Ni2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 4-(2-methyl-1,3-thiazol-4-yl)benzene-1,2-diol | |
0.079 | - |
after removal of divalent metal ions activated with 0.02 mM Mn2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 4-(2-methyl-1,3-thiazol-4-yl)benzene-1,2-diol | |
0.094 | - |
after removal of divalent metal ions activated with 0.0025 mM Fe2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-[(2,4-dichlorobenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | |
0.103 | - |
after removal of divalent metal ions activated with 0.02 mM Mn2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-[(4-methylbenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | |
0.128 | - |
after removal of divalent metal ions activated with 0.0025 mM Fe2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-[(2,4-dichlorobenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | |
0.145 | - |
after removal of divalent metal ions activated with 0.02 mM Mn2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-[(4-methylbenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | |
0.186 | - |
after removal of divalent metal ions activated with 0.02 mM Ni2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-(2-chlorophenyl)furan-2-carboxylic acid | |
0.188 | - |
after removal of divalent metal ions activated with 0.005 mM Ni2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-(2-chlorophenyl)furan-2-carboxylic acid | |
0.251 | - |
after removal of divalent metal ions activated with 0.005 mM Ni2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-(2,5-dichlorophenyl)furan-2-carboxylic acid | |
0.286 | - |
after removal of divalent metal ions activated with 0.02 mM Ni2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-(2,5-dichlorophenyl)furan-2-carboxylic acid | |
0.288 | - |
after removal of divalent metal ions activated with 0.0025 mM Fe2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-(2-chlorophenyl)furan-2-carboxylic acid | |
0.303 | - |
after removal of divalent metal ions activated with 0.02 mM Mn2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-[(4-fluorobenzyl)sulfanyl]-4H-1,2,4-triazol-3-amine | |
0.355 | - |
after removal of divalent metal ions activated with 0.0025 mM Fe2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-(2,5-dichlorophenyl)furan-2-carboxylic acid | |
0.358 | - |
after removal of divalent metal ions activated with 0.0025 mM Fe2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-(2-chlorophenyl)furan-2-carboxylic acid | |
0.381 | - |
after removal of divalent metal ions activated with 0.02 mM Co2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-(2-chlorophenyl)furan-2-carboxylic acid | |
0.394 | - |
after removal of divalent metal ions activated with 0.02 mM Co2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-(2-chlorophenyl)furan-2-carboxylic acid | |
0.494 | - |
after removal of divalent metal ions activated with 0.02 mM Co2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | 5-(2,5-dichlorophenyl)furan-2-carboxylic acid |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00033 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.020 mM Mn2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.000479 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.020 mM Mn2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.000948 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.020 mM Co2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.00105 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.020 mM Co2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.0017 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.0025 mM Fe2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.00217 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.020 mM Ni2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.00275 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.005 mM Ni2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii | |
0.00278 | - |
Met-4-methylcoumaryl-7-amide | after removal of divalent metal ions activated with 0.0025 mM Fe2+, pH not specified in the publication, temperature not specified in the publication | Acinetobacter baumannii |