Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
((2RS,3R)-3-amino-2-hydroxy-5-ethylthio)pentanoyl-((S)-(-)-(1-naphthyl)ethyl)amide | A-311263, the inhibitor has little or no preference for the type of divalent metal ion that occupies the active site of MetAP | Escherichia coli | |
((2RS,3R)-3-amino-2-hydroxy-5-ethylthio)pentanoyl-((S)-(-)-(1-naphthyl)ethyl)amide | A-311263, the inhibitor has little or no preference for the type of divalent metal ion that occupies the active site of MetAP | Pyrococcus furiosus | |
3-((2-naphthylmethyl)sulfanyl)-4H-1,2,4-triazole | A-310840, the inhibitor binds to the Co2+-loaded enzyme well but is approximately 23fold less potent for Mn2+-loaded MetAP | Escherichia coli | |
3-((2-naphthylmethyl)sulfanyl)-4H-1,2,4-triazole | A-310840 | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activity requires divalent cations like Co2+ | Escherichia coli | |
Co2+ | activity requires divalent cations like Co2+ | Pyrococcus furiosus | |
Mn2+ | activity requires divalent cations like Mn2+ | Escherichia coli | |
Mn2+ | activity requires divalent cations like Mn2+ | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Pyrococcus furiosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Met-Gly-L-Met-L-Met + H2O | - |
Escherichia coli | L-Met + Gly-L-Met-L-Met | - |
? | |
L-Met-Gly-L-Met-L-Met + H2O | - |
Pyrococcus furiosus | L-Met + Gly-L-Met-L-Met | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MetAP-I | - |
Escherichia coli |
MetAP-II | - |
Pyrococcus furiosus |
methionine aminopeptidase type I | - |
Escherichia coli |
methionine aminopeptidase type II | - |
Pyrococcus furiosus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
((2RS,3R)-3-amino-2-hydroxy-5-ethylthio)pentanoyl-((S)-(-)-(1-naphthyl)ethyl)amide | Co2+-loaded wild type enzyme, in 25 mM HEPES buffer, pH 7.5, containing 150 mM KCl, at 30°C | Pyrococcus furiosus | |
0.003 | - |
3-((2-naphthylmethyl)sulfanyl)-4H-1,2,4-triazole | Co2+-loaded wild type enzyme, in 25 mM HEPES buffer, pH 7.5, containing 150 mM KCl, at 30°C | Pyrococcus furiosus | |
0.003 | - |
((2RS,3R)-3-amino-2-hydroxy-5-ethylthio)pentanoyl-((S)-(-)-(1-naphthyl)ethyl)amide | Co2+-loaded wild type enzyme, in 25 mM HEPES buffer, pH 7.5, containing 150 mM KCl, at 30°C | Escherichia coli | |
0.008 | - |
3-((2-naphthylmethyl)sulfanyl)-4H-1,2,4-triazole | Co2+-loaded wild type enzyme, in 25 mM HEPES buffer, pH 7.5, containing 150 mM KCl, at 30°C | Escherichia coli | |
0.02 | - |
((2RS,3R)-3-amino-2-hydroxy-5-ethylthio)pentanoyl-((S)-(-)-(1-naphthyl)ethyl)amide | Mn2+-loaded wild type enzyme, in 25 mM HEPES buffer, pH 7.5, containing 150 mM KCl, at 30°C | Escherichia coli | |
0.02 | - |
((2RS,3R)-3-amino-2-hydroxy-5-ethylthio)pentanoyl-((S)-(-)-(1-naphthyl)ethyl)amide | Mn2+-loaded wild type enzyme, in 25 mM HEPES buffer, pH 7.5, containing 150 mM KCl, at 30°C | Pyrococcus furiosus | |
0.44 | - |
3-((2-naphthylmethyl)sulfanyl)-4H-1,2,4-triazole | Mn2+-loaded wild type enzyme, in 25 mM HEPES buffer, pH 7.5, containing 150 mM KCl, at 30°C | Pyrococcus furiosus | |
0.75 | - |
3-((2-naphthylmethyl)sulfanyl)-4H-1,2,4-triazole | Mn2+-loaded wild type enzyme, in 25 mM HEPES buffer, pH 7.5, containing 150 mM KCl, at 30°C | Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.015 | - |
Co2+-loaded wild type enzyme, in 25 mM HEPES buffer, pH 7.5, containing 150 mM KCl, at 30°C | Pyrococcus furiosus | 3-((2-naphthylmethyl)sulfanyl)-4H-1,2,4-triazole | |
0.02 | - |
Co2+-loaded wild type enzyme, in 25 mM HEPES buffer, pH 7.5, containing 150 mM KCl, at 30°C | Pyrococcus furiosus | ((2RS,3R)-3-amino-2-hydroxy-5-ethylthio)pentanoyl-((S)-(-)-(1-naphthyl)ethyl)amide | |
0.027 | - |
Mn2+-loaded wild type enzyme, in 25 mM HEPES buffer, pH 7.5, containing 150 mM KCl, at 30°C | Pyrococcus furiosus | ((2RS,3R)-3-amino-2-hydroxy-5-ethylthio)pentanoyl-((S)-(-)-(1-naphthyl)ethyl)amide | |
0.032 | - |
Co2+-loaded wild type enzyme, in 25 mM HEPES buffer, pH 7.5, containing 150 mM KCl, at 30°C | Escherichia coli | ((2RS,3R)-3-amino-2-hydroxy-5-ethylthio)pentanoyl-((S)-(-)-(1-naphthyl)ethyl)amide | |
0.034 | - |
Mn2+-loaded wild type enzyme, in 25 mM HEPES buffer, pH 7.5, containing 150 mM KCl, at 30°C | Escherichia coli | ((2RS,3R)-3-amino-2-hydroxy-5-ethylthio)pentanoyl-((S)-(-)-(1-naphthyl)ethyl)amide | |
0.044 | - |
Co2+-loaded wild type enzyme, in 25 mM HEPES buffer, pH 7.5, containing 150 mM KCl, at 30°C | Escherichia coli | 3-((2-naphthylmethyl)sulfanyl)-4H-1,2,4-triazole | |
1 | - |
Mn2+-loaded wild type enzyme, in 25 mM HEPES buffer, pH 7.5, containing 150 mM KCl, at 30°C | Escherichia coli | 3-((2-naphthylmethyl)sulfanyl)-4H-1,2,4-triazole | |
1 | - |
Mn2+-loaded wild type enzyme, in 25 mM HEPES buffer, pH 7.5, containing 150 mM KCl, at 30°C | Pyrococcus furiosus | 3-((2-naphthylmethyl)sulfanyl)-4H-1,2,4-triazole |