Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli AS19 and BL21(DE3) cells | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(2-methyl-1,3-thiazol-4-yl)benzene-1,2-diol | does only inhibit Fe2+-activated MetAP enzyme, but not Mn2+- or Co2+-activated MetAP enzymes | Escherichia coli | |
4-(3-methylthiophen-2-yl)benzene-1,2-diol | does only inhibit Fe2+-activated MetAP enzyme, but not Mn2+- or Co2+-activated MetAP enzymes | Escherichia coli | |
5-(2,5-dichlorophenyl)furan-2-carboxylic acid | - |
Escherichia coli | |
5-(2-chlorophenyl)furan-2-carboxylic acid | - |
Escherichia coli | |
N-(pyridin-2-ylmethyl)-N'-1,3-thiazol-2-ylethanediamide | - |
Escherichia coli | |
N-cyclopentyl-N'-1,3-thiazol-2-ylethanediamide | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | Co2+ also activates | Escherichia coli | |
Fe2+ | native cofactor | Escherichia coli | |
Mn2+ | Mn2+ also activates | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
strain ATCC 25922 | - |
Purification (Comment) | Organism |
---|---|
GSTrap HP column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Met-7-amido-4-methylcoumarin + H2O | - |
Escherichia coli | L-Met + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MetAP | - |
Escherichia coli |
methionine aminopeptidase | - |
Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.008 | - |
using Fe2+-activated MetAP enzyme | Escherichia coli | 4-(3-methylthiophen-2-yl)benzene-1,2-diol | |
0.0131 | - |
using Fe2+-activated MetAP enzyme | Escherichia coli | 4-(2-methyl-1,3-thiazol-4-yl)benzene-1,2-diol | |
0.023 | - |
using Mn2+-activated MetAP enzyme | Escherichia coli | 4-(3-methylthiophen-2-yl)benzene-1,2-diol | |
0.041 | - |
using Co2+-activated MetAP enzyme | Escherichia coli | 4-(3-methylthiophen-2-yl)benzene-1,2-diol |