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Literature summary for 3.4.11.18 extracted from

  • Chai, S.C.; Wang, W.L.; Ye, Q.Z.
    FE(II) is the native cofactor for Escherichia coli methionine aminopeptidase (2008), J. Biol. Chem., 283, 26879-26885.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli AS19 and BL21(DE3) cells Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
4-(2-methyl-1,3-thiazol-4-yl)benzene-1,2-diol does only inhibit Fe2+-activated MetAP enzyme, but not Mn2+- or Co2+-activated MetAP enzymes Escherichia coli
4-(3-methylthiophen-2-yl)benzene-1,2-diol does only inhibit Fe2+-activated MetAP enzyme, but not Mn2+- or Co2+-activated MetAP enzymes Escherichia coli
5-(2,5-dichlorophenyl)furan-2-carboxylic acid
-
Escherichia coli
5-(2-chlorophenyl)furan-2-carboxylic acid
-
Escherichia coli
N-(pyridin-2-ylmethyl)-N'-1,3-thiazol-2-ylethanediamide
-
Escherichia coli
N-cyclopentyl-N'-1,3-thiazol-2-ylethanediamide
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ Co2+ also activates Escherichia coli
Fe2+ native cofactor Escherichia coli
Mn2+ Mn2+ also activates Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
strain ATCC 25922
-

Purification (Commentary)

Purification (Comment) Organism
GSTrap HP column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Met-7-amido-4-methylcoumarin + H2O
-
Escherichia coli L-Met + 7-amino-4-methylcoumarin
-
?

Synonyms

Synonyms Comment Organism
MetAP
-
Escherichia coli
methionine aminopeptidase
-
Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.008
-
using Fe2+-activated MetAP enzyme Escherichia coli 4-(3-methylthiophen-2-yl)benzene-1,2-diol
0.0131
-
using Fe2+-activated MetAP enzyme Escherichia coli 4-(2-methyl-1,3-thiazol-4-yl)benzene-1,2-diol
0.023
-
using Mn2+-activated MetAP enzyme Escherichia coli 4-(3-methylthiophen-2-yl)benzene-1,2-diol
0.041
-
using Co2+-activated MetAP enzyme Escherichia coli 4-(3-methylthiophen-2-yl)benzene-1,2-diol