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Literature summary for 3.4.11.18 extracted from

  • Mitra, S.; Job, K.M.; Meng, L.; Bennett, B.; Holz, R.C.
    Analyzing the catalytic role of Asp97 in the methionine aminopeptidase from Escherichia coli (2008), FEBS J., 275, 6248-6259.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.18

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes are expressed in Escherichia coli BL21 Star(DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
D97A the mutation causes an about 9000fold decrease in kcat and a 6100fold decreased catalytic efficiency (kcat/Km) for the Co(II)-loaded enzyme towards L-Met-Gly-L-Met-L-Met as compared to the wild type enzyme, the mutation effectively removes the ability of the enzyme to form a dinuclear site, providing a MetAP enzyme that retains catalytic activity, albeit at extremely low levels Escherichia coli
D97E the mutation causes an about 9000fold decrease in kcat and a 2000fold decreased catalytic efficiency (kcat/Km) for the Co(II)-loaded enzyme towards L-Met-Gly-L-Met-L-Met as compared to the wild type enzyme Escherichia coli
D97N the mutation causes an about 9000fold decrease in kcat and a 3000fold decreased catalytic efficiency (kcat/Km) for the Co(II)-loaded enzyme towards L-Met-Gly-L-Met-L-Met as compared to the wild type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 L-Met-Gly-L-Met-L-Met mutant enzyme D97N, Co2+-loaded, in 25 mM HEPES buffer, at 30°C and pH 7.5 Escherichia coli
1.1
-
 L-Met-Gly-L-Met-L-Met mutant enzyme D97A, Co2+-loaded, in 25 mM HEPES buffer, at 30°C and pH 7.5 Escherichia coli
1.8
-
 L-Met-Gly-L-Met-L-Met mutant enzyme D97E, Co2+-loaded, in 25 mM HEPES buffer, at 30°C and pH 7.5 Escherichia coli
3
-
 L-Met-Gly-L-Met-L-Met wild type enzyme, Co2+-loaded, in 25 mM HEPES buffer, at 30°C and pH 7.5 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the enzyme contains one Co2+ ion Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29630
-
 purified enzyme, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AE18
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Met-Gly-L-Met-L-Met + H2O
-
 Escherichia coli L-Met + Gly-L-Met-L-Met
-
 ?

Synonyms

Synonyms Comment Organism
MetAP-I
-
 Escherichia coli
type I MetAP
-
 Escherichia coli
type I methionine aminopeptidase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 L-Met-Gly-L-Met-L-Met mutant enzyme D97N, Co2+-loaded, in 25 mM HEPES buffer, at 30°C and pH 7.5 Escherichia coli
0.002
-
 L-Met-Gly-L-Met-L-Met mutant enzyme D97E, Co2+-loaded, in 25 mM HEPES buffer, at 30°C and pH 7.5 Escherichia coli
0.003
-
 L-Met-Gly-L-Met-L-Met mutant enzyme D97A, Co2+-loaded, in 25 mM HEPES buffer, at 30°C and pH 7.5 Escherichia coli
18.3
-
 L-Met-Gly-L-Met-L-Met wild type enzyme, Co2+-loaded, in 25 mM HEPES buffer, at 30°C and pH 7.5 Escherichia coli