Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.11.18 extracted from

  • Huang, M.; Xie, S.X.; Ma, Z.Q.; Huang, Q.Q.; Nan, F.J.; Ye, Q.Z.
    Inhibition of monometalated methionine aminopeptidase: inhibitor discovery and crystallographic analysis (2007), J. Med. Chem., 50, 5735-5742.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.18

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with inhibitor, hanging drop vapour diffusion method with 10-15% PEG 20 000, 0.1 M MES (pH 6.5) and 0.2 mM or 0.8 mM MnCl2 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
5-(2-chlorophenyl)furan-2-carbohydrazide
-
 Escherichia coli
5-(2-chlorophenyl)furan-2-carboxylic acid
-
 Escherichia coli
imidazo[2,1-a]isoquinoline-2-carbohydrazide
-
 Escherichia coli
norleucine phosphonate
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AE18
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine-7-amido-4-methylcoumarin + H2O
-
 Escherichia coli L-methionine + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
MetAP
-
 Escherichia coli
methionine aminopeptidase
-
 Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00051
-
 in the presence of 0.1 mM Mn2+ Escherichia coli 5-(2-chlorophenyl)furan-2-carboxylic acid
0.00064
-
 in the presence of 0.005 mM Mn2+ Escherichia coli imidazo[2,1-a]isoquinoline-2-carbohydrazide
0.00078
-
 in the presence of 0.005 mM Mn2+ Escherichia coli 5-(2-chlorophenyl)furan-2-carboxylic acid
0.00116
-
 in the presence of 0.005 mM Mn2+ Escherichia coli 5-(2-chlorophenyl)furan-2-carbohydrazide
0.00244
-
 in the presence of 0.001 mM Co2+ Escherichia coli imidazo[2,1-a]isoquinoline-2-carbohydrazide
0.00431
-
 in the presence of 0.1 mM Mn2+ Escherichia coli imidazo[2,1-a]isoquinoline-2-carbohydrazide
0.00578
-
 in the presence of 0.01 mM Ni2+ Escherichia coli 5-(2-chlorophenyl)furan-2-carbohydrazide
0.00615
-
 in the presence of 0.01 mM Ni2+ Escherichia coli imidazo[2,1-a]isoquinoline-2-carbohydrazide
0.00656
-
 in the presence of 0.1 mM Mn2+ Escherichia coli 5-(2-chlorophenyl)furan-2-carbohydrazide
0.0174
-
 in the presence of 0.006 mM Fe2+ Escherichia coli imidazo[2,1-a]isoquinoline-2-carbohydrazide
0.0278
-
 in the presence of 0.1 mM Co2+ Escherichia coli imidazo[2,1-a]isoquinoline-2-carbohydrazide
0.058
-
 in the presence of 0.006 mM Fe2+ Escherichia coli 5-(2-chlorophenyl)furan-2-carbohydrazide
0.0771
-
 in the presence of 0.1 mM Co2+ Escherichia coli 5-(2-chlorophenyl)furan-2-carbohydrazide
0.116
-
 in the presence of 0.006 mM Fe2+ Escherichia coli 5-(2-chlorophenyl)furan-2-carboxylic acid
0.138
-
 in the presence of 0.1 mM Co2+ Escherichia coli 5-(2-chlorophenyl)furan-2-carboxylic acid
0.141
-
 in the presence of 0.01 mM Ni2+ Escherichia coli 5-(2-chlorophenyl)furan-2-carboxylic acid
0.2
-
 IC50 above 0.2 mM, in the presence of 0.001 mM Co2+ Escherichia coli 5-(2-chlorophenyl)furan-2-carboxylic acid