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Literature summary for 3.4.11.18 extracted from

  • Walker, K.W.; Yi, E.; Bradshaw, R.A.
    Yeast (Saccharomyces cerevisiae) methionine aminopeptidase I: rapid purification and improved activity assay (1999), Biotechnol. Appl. Biochem., 29, 157-163.
    View publication on PubMed
Search for more literature for 3.4.11.18

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 cells, Pichia pastoris and Saccharomyces cerevisiae, glutathione S-transferase-Met AP I fusion protein Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.139
-
 Met-Ala-Ser-Trp pH 7.25, 30°C Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43779
-
 x * 43779, MALDI-MS Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Met-peptide + H2O Saccharomyces cerevisiae the enzyme is responsible for hydrolysis of the initiator methionine residue from the majority of newly synthesized proteins. Potentially important role in the production of recombinant proteins, since failure to correctly remove initiator methionine residues can result in a product that is inactive or immunogenic Met + peptide
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Met-Ala-Ser-Trp + H2O
-
 Saccharomyces cerevisiae Met + Ala-Ser-Trp
-
 ?
Met-peptide + H2O the enzyme is responsible for hydrolysis of the initiator methionine residue from the majority of newly synthesized proteins. Potentially important role in the production of recombinant proteins, since failure to correctly remove initiator methionine residues can result in a product that is inactive or immunogenic Saccharomyces cerevisiae Met + peptide
-
 ?
Met-Val-Ser-His-Arg-Trp-Asp-Trp + H2O
-
 Saccharomyces cerevisiae Met + Val-Ser-His-Arg-Trp-Asp-Trp
-
 ?

Subunits

Subunits Comment Organism
? x * 43779, MALDI-MS Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.33
-
 Met-Val-Ser-His-Arg-Trp-Asp-Trp pH 7.25, 30°C Saccharomyces cerevisiae
8.45
-
 Met-Ala-Ser-Trp pH 7.25, 30°C Saccharomyces cerevisiae