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Literature summary for 3.4.11.18 extracted from

  • Chung, J.M.; Chung, I.Y.; Lee, Y.S.
    The purification and characterization of a Bacillus stearothermophilus methionine aminopeptidase (MetAP) (2002), J. Biochem. Mol. Biol., 35, 228-235.
    View publication on PubMed
Search for more literature for 3.4.11.18

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Geobacillus stearothermophilus
Ca2+ 50% inhibition at 2 mM, 52% inhibition at 4 mM Geobacillus stearothermophilus
Cu2+ 52% inhibition at 2 mM, 88% inhibition at 4 mM Geobacillus stearothermophilus
EDTA
-
 Geobacillus stearothermophilus
Fe2+ 1 mM, complete inhibition Geobacillus stearothermophilus
Hg2+ 1 mM, complete inhibition Geobacillus stearothermophilus
Mn2+ 1 mM, complete inhibition Geobacillus stearothermophilus
Ni2+ 1 mM, complete inhibition Geobacillus stearothermophilus
Zn2+ 71% inhibition at 5 mM Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3
-
 L-Met-L-Ala-L-Ser pH 7.4 Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ Co2+-dependent metallopeptidase Geobacillus stearothermophilus
Mg2+ slight activation Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 2 * 41000, SDS-PAGE Geobacillus stearothermophilus
81300
-
 gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Met-peptide + H2O Geobacillus stearothermophilus the enzyme catalyzes the removal of an amino-terminal methionine from a newly synthesized polypeptide Met + peptide
-
 ?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Met-L-Ala-L-Ser + H2O
-
 Geobacillus stearothermophilus L-Met + L-Ala-L-Ser
-
 ?
Leu-Gly-Gly + H2O 34% of the activity with Met-Ala-Ser Geobacillus stearothermophilus ?
-
 ?
Leu-Leu-Tyr + H2O 32% of the activity with Leu-Gly-Gly Geobacillus stearothermophilus ?
-
 ?
Leu-Ser-Phe + H2O 30% of the activity with Met-Ala-Ser Geobacillus stearothermophilus ?
-
 ?
Met-peptide + H2O the enzyme catalyzes the removal of an amino-terminal methionine from a newly synthesized polypeptide Geobacillus stearothermophilus Met + peptide
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 41000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80 85
-
 Geobacillus stearothermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 100 50°C: about 45% of maximal activity, 100: about 20% of maximal activity Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 45 min, stable Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.3 pH 6.5: about 35% of maximal activity, pH 9.3: about 75% of maximal activity Geobacillus stearothermophilus

pI Value

Organism Comment pI Value Maximum pI Value
Geobacillus stearothermophilus isoelectric focusing
-
 4.1