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Literature summary for 3.4.11.17 extracted from

  • Adachi, O.
    Tryptophanyl aminopeptidase (2004), Handbook of Proteolytic Enzymes (Barrett, A.J., Rawlings, N.D., Woessner, J.F., eds), 2nd. ed., 1014-1015.
No PubMed abstract available
Search for more literature for 3.4.11.17

Application

Application Comment Organism
synthesis the enzyme is useful for synthesis of L-Trp because it is not inhibited by high levels of the product Cutaneotrichosporon cutaneum

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Cutaneotrichosporon cutaneum

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl
-
 Cutaneotrichosporon cutaneum
N-ethylmaleimide
-
 Cutaneotrichosporon cutaneum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Cutaneotrichosporon cutaneum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 25% of the activation caused by Mn2+ Cutaneotrichosporon cutaneum
Mn2+ above 2.5 mM, required Cutaneotrichosporon cutaneum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 4 * 68000, SDS-PAGE Cutaneotrichosporon cutaneum
270000
-
 gel filtration Cutaneotrichosporon cutaneum

Organism

Organism UniProt Comment Textmining
Cutaneotrichosporon cutaneum
-
 IFO 0173
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cutaneotrichosporon cutaneum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-amide + H2O 14% of the activity with Trp-amide Cutaneotrichosporon cutaneum Ala + NH3
-
 ?
Asn + H2O 18% of the activity with Trp-amide Cutaneotrichosporon cutaneum Asp + NH3
-
 ?
Gln + H2O 18% of the activity with Trp-amide Cutaneotrichosporon cutaneum Glu + NH3
-
 ?
L-citrulline + H2O 22% of the activity with Trp-amide Cutaneotrichosporon cutaneum (2S)-2-amino-5-(carboxyamino)pentanoic acid + NH3
-
 ?
Leu-amide + H2O 26% of the activity with Trp-amide Cutaneotrichosporon cutaneum Leu + NH3
-
 ?
Met-amide + H2O 14% of the activity with Trp-amide Cutaneotrichosporon cutaneum Met + NH3
-
 ?
Phe-amide + H2O 47% of the activity with Trp-amide Cutaneotrichosporon cutaneum Phe + NH3
-
 ?
Ser-amide + H2O 7% of the activity with Trp-amide Cutaneotrichosporon cutaneum Ser + NH3
-
 ?
Trp-amide + H2O
-
 Cutaneotrichosporon cutaneum Trp + NH3
-
 ?
Tyr-amide + H2O 22% of the activity with Trp-amide Cutaneotrichosporon cutaneum Tyr + NH3
-
 ?
Val-amide + H2O 15% of the activity with Trp-amide Cutaneotrichosporon cutaneum Val + NH3
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 68000, SDS-PAGE Cutaneotrichosporon cutaneum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45 at pH 7.5 Cutaneotrichosporon cutaneum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 10 min, stable up to Cutaneotrichosporon cutaneum
60
-
 heating above causes rapid inactivation Cutaneotrichosporon cutaneum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 9.5
-
 Cutaneotrichosporon cutaneum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 8.5 20 h, stable at room temperature Cutaneotrichosporon cutaneum

pI Value

Organism Comment pI Value Maximum pI Value
Cutaneotrichosporon cutaneum isoelectric focusing
-
 4.7