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Literature summary for 3.4.11.16 extracted from

  • Gee, N.S.; Kenny, A.J.
    Proteins of the kidney microvillar membrane. Enzymic and molecular properties of aminopeptidase W (1987), Biochem. J., 246, 97-102.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 50% inhibition at 0.6 mM Sus scrofa
amastatin 50% inhibition at 0.002 mM Sus scrofa
bestatin 50% inhibition at 0.006 mM Sus scrofa
captopril 50% inhibition at 0.3 mM Sus scrofa
Co2+ 65-90% inhibition at 1 mM Sus scrofa
Cu2+ 65-90% inhibition at 1 mM Sus scrofa
dithiothreitol 50% inhibition at 0.1 mM Sus scrofa
additional information many group-specific inhibitors for cysteine proteases have no effect Sus scrofa
Ni2+ 25% inhibition at 1 mM Sus scrofa
phosphate
-
Sus scrofa
Zn2+ 65-90% inhibition at 1 mM, reactivated at 10 mM EDTA Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.488
-
Leu-Trp
-
Sus scrofa
0.568
-
Glu-Trp
-
Sus scrofa
0.625
-
Ala-Trp
-
Sus scrofa
1.7
-
Pro-Trp
-
Sus scrofa
1.757
-
Gly-Trp
-
Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
microvillus
-
Sus scrofa 5902
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ 1.2 mol zinc per mol enzyme, probably involved in catalysis Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Peptides + H2O Sus scrofa involved in intestinal digestion of small, pre-digested peptide fragments ?
-
ir

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 31% carbohydrates Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
Sus scrofa
-
kidney
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity highly buffer-dependent, optimal in 100 mM Tris-HCl Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Trp + H2O
-
Sus scrofa Ala + Trp
-
ir
Arg-Trp + H2O
-
Sus scrofa Arg + Trp
-
ir
Asp-Phe + H2O
-
Sus scrofa Asp + Phe
-
ir
Glu-Trp + H2O
-
Sus scrofa Glu + Trp
-
ir
Glu-Tyr + H2O
-
Sus scrofa Glu + Tyr
-
ir
Gly-Trp + H2O
-
Sus scrofa Gly + Trp
-
ir
Gly-Tyr + H2O
-
Sus scrofa Gly + Tyr
-
ir
Ile-Phe + H2O
-
Sus scrofa Ile + Phe
-
ir
Ile-Trp + H2O
-
Sus scrofa Ile + Trp
-
ir
Leu-Phe + H2O
-
Sus scrofa Leu + Phe
-
ir
Leu-Trp + H2O
-
Sus scrofa Leu + Trp
-
ir
Leu-Tyr + H2O
-
Sus scrofa Leu + Tyr
-
ir
Peptides + H2O involved in intestinal digestion of small, pre-digested peptide fragments Sus scrofa ?
-
ir
Phe-Phe + H2O
-
Sus scrofa Phe + Phe
-
ir
Phe-Trp + H2O
-
Sus scrofa Phe + Trp
-
ir
Phe-Tyr + H2O
-
Sus scrofa Phe + Tyr
-
ir
Pro-Trp + H2O
-
Sus scrofa Pro + Trp
-
ir
Trp-Phe + H2O
-
Sus scrofa Trp + Phe
-
ir
Trp-Trp + H2O
-
Sus scrofa Trp + Trp
-
ir
Tyr-Leu + H2O
-
Sus scrofa Tyr + Leu
-
ir
Tyr-Phe + H2O
-
Sus scrofa Tyr + Phe
-
ir
Val-Trp + H2O
-
Sus scrofa Val + Trp
-
ir

Synonyms

Synonyms Comment Organism
aminopeptidase W
-
Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
29.6
-
Pro-Trp
-
Sus scrofa
38.5
-
Ala-Trp
-
Sus scrofa
92.6
-
Leu-Trp
-
Sus scrofa
113
-
Glu-Trp
-
Sus scrofa
127
-
Gly-Trp
-
Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Sus scrofa