Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activation of 75000 Da-proform to 70000 Da active form by protease B | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 95% inhibition at 1 mM | Saccharomyces cerevisiae | |
amastatin | 90% inhibition at 0.02 mM | Saccharomyces cerevisiae | |
bestatin | 48% inhibition at 0.02 mM | Saccharomyces cerevisiae | |
Cu2+ | 90% inhibition at 0.5 mM | Saccharomyces cerevisiae | |
diphosphate | 93% inhibition at 1 mM | Saccharomyces cerevisiae | |
dithiothreitol | 94% inhibition at 1 mM | Saccharomyces cerevisiae | |
EDTA | 99% inhibition at 1 mM | Saccharomyces cerevisiae | |
EGTA | 99.9% inhibition at 1 mM | Saccharomyces cerevisiae | |
Mn2+ | 67% inhibition at 0.5 mM | Saccharomyces cerevisiae | |
p-chloromercuribenzoic acid | 81% inhibition at 0.1 mM | Saccharomyces cerevisiae | |
Triphosphate | 99.9% inhibition at 1 mM | Saccharomyces cerevisiae | |
Zn2+ | 0.81-0.84 mol per mol enzyme; 72% inhibition at 0.5 mM | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
autophagic vacuole | - |
Saccharomyces cerevisiae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | required | Saccharomyces cerevisiae | |
Co2+ | 800-900% activation by 0.5 mM | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
1 * 70000, SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
2 molecular forms | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Saccharomyces cerevisiae |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate-dependent | Saccharomyces cerevisiae |
0.028 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Arg)3 + H2O | - |
Saccharomyces cerevisiae | Arg + Arg-Arg | - |
ir | |
(Leu)3 + H2O | - |
Saccharomyces cerevisiae | Leu + Leu-Leu | - |
ir | |
(Lys)3 + H2O | - |
Saccharomyces cerevisiae | Lys + Lys-Lys | - |
ir | |
Ala-7-amido-4-methylcoumarin + H2O | - |
Saccharomyces cerevisiae | Ala + 7-amino-4-methylcoumarin | - |
ir | |
Arg-7-amido-4-methylcoumarin + H2O | - |
Saccharomyces cerevisiae | Arg + 7-amino-4-methylcoumarin | - |
ir | |
Arg-Arg-4-methylcoumaryl-7-amide + H2O | - |
Saccharomyces cerevisiae | Arg + Arg-4-methylcoumaryl-7-amide | - |
ir | |
Arg-Arg-Lys-Ala-Leu-Ser-Pro + H2O | - |
Saccharomyces cerevisiae | Arg + Arg-Lys-Ala-Leu-Ser-Pro | - |
ir | |
Arg-Gly-Tyr-Ser-Leu-Gly + H2O | - |
Saccharomyces cerevisiae | Arg + Gly-Tyr-Ser-Leu-Gly | - |
ir | |
Arg-Val + H2O | - |
Saccharomyces cerevisiae | Arg + Val | - |
ir | |
Arg-Val-OMe + H2O | - |
Saccharomyces cerevisiae | Arg + Val-OMe | - |
ir | |
Arg-Val-Tyr-Ile-His-Pro-Phe + H2O | - |
Saccharomyces cerevisiae | Arg + Val-Tyr-Ile-His-Pro-Phe | - |
ir | |
Leu-7-amido-4-methylcoumarin + H2O | - |
Saccharomyces cerevisiae | Leu + 7-amino-4-methylcoumarin | - |
ir | |
Leu-Arg-Arg-Ala-Ser-Leu-Gly + H2O | - |
Saccharomyces cerevisiae | Leu + Arg-Arg-Ala-Ser-Leu-Gly | - |
ir | |
Leu-Leu + H2O | - |
Saccharomyces cerevisiae | Leu + Leu | - |
ir | |
Leu-Leu-OMe + H2O | - |
Saccharomyces cerevisiae | Leu + Leu-OMe | - |
ir | |
Lys-7-amido-4-methylcoumarin + H2O | - |
Saccharomyces cerevisiae | Lys + 7-amino-4-methylcoumarin | - |
ir | |
Lys-Ala-4-methylcoumaryl-7-amide + H2O | - |
Saccharomyces cerevisiae | Lys + Ala-4-methylcoumaryl-7-amide | - |
ir | |
Met-7-amido-4-methylcoumarin + H2O | - |
Saccharomyces cerevisiae | Met + 7-amino-4-methylcoumarin | - |
ir | |
Met-Ala-Ser + H2O | - |
Saccharomyces cerevisiae | Met + Ala-Ser | - |
ir | |
Met-Arg-Phe + H2O | - |
Saccharomyces cerevisiae | Met + Arg-Phe | - |
ir | |
Met-Gly-Gly + H2O | - |
Saccharomyces cerevisiae | Met + Gly-Gly | - |
ir | |
additional information | generally higher activities with dipeptide-4-methylcoumaryl-7-amides than with amino-acid-4-methylcoumaryl-7-amides | Saccharomyces cerevisiae | ? | - |
? | |
Pro-Phe-Arg-4-methylcoumaryl-7-amide + H2O | - |
Saccharomyces cerevisiae | Pro + Phe-Arg-4-methylcoumaryl-7-amide | - |
ir | |
Ser-7-amido-4-methylcoumarin + H2O | - |
Saccharomyces cerevisiae | Ser + 7-amino-4-methylcoumarin | - |
ir | |
Tyr-Gly-Gly-Phe-Leu + H2O | - |
Saccharomyces cerevisiae | Tyr + Gly-Gly-Phe-Leu | - |
ir |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 70000, SDS-PAGE | Saccharomyces cerevisiae |