Any feedback?
Literature summary for 3.4.11.13 extracted from
- A novel aminopeptidase from Clostridium histolyticum (1973), Biochem. Biophys. Res. Commun., 50, 405-412.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Hathewaya histolytica |  |
| p-mercuribenzoate | - |
Hathewaya histolytica | |
| Zn2+ | - |
Hathewaya histolytica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Hathewaya histolytica |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Hathewaya histolytica | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Hathewaya histolytica | |
| Co2+ | - |
Hathewaya histolytica | |
| Mn2+ | - |
Hathewaya histolytica | |
| Ni2+ | - |
Hathewaya histolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 340000 | - |
gel filtration | Hathewaya histolytica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Peptides + H2O | Hathewaya histolytica | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hathewaya histolytica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hathewaya histolytica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.5 | - |
- |
Hathewaya histolytica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.05 M phosphate buffer or 0.05 M Na-acetate buffer, pH 5.6, 1 mM EDTA, several years, little loss of activity, can be restored by treatment with 2.5 mM dithiothreitol | Hathewaya histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (Ala)3 + H2O | - |
Hathewaya histolytica | Ala + Ala-Ala | - |
ir | |
| (Ala)4 + H2O | - |
Hathewaya histolytica | Ala + (Ala)3 | or Ala-Ala + Ala-Ala | ir | |
| (Ala)4-NH2 + H2O | - |
Hathewaya histolytica | Ala + (Ala)3-NH2 | or Ala-Ala + Ala-Ala-NH2 or (Ala)3 + Ala-NH2 | ir | |
| (Pro-Gly-Pro)4-6 + H2O | - |
Hathewaya histolytica | Pro + Gly-Pro-(Pro-Gly-Pro)3-5 | - |
ir | |
| (Pro-Gly-Pro)4-6-OMe + H2O | - |
Hathewaya histolytica | Pro + Gly-Pro-(Pro-Gly-Pro)3-5-OMe | - |
ir | |
| Ala-Ala-Phe-Ala + H2O | - |
Hathewaya histolytica | Ala + Ala-Phe-Ala | or Ala-Ala + Phe-Ala | ir | |
| Ala-Gly-Gly + H2O | - |
Hathewaya histolytica | Ala + Gly-Gly | - |
ir | |
| Ala-Lys + H2O | - |
Hathewaya histolytica | Ala + Lys | - |
ir | |
| Gly-Gly-Phe + H2O | - |
Hathewaya histolytica | Gly + Gly-Phe | - |
ir | |
| Gly-Gly-Phe-Ala + H2O | - |
Hathewaya histolytica | Gly + Gly-Phe-Ala | or Gly-Gly + Phe-Ala | ir | |
| Gly-Phe-Ala + H2O | - |
Hathewaya histolytica | Gly + Phe-Ala | - |
ir | |
| Hyp-Ala-NH2 + H2O | - |
Hathewaya histolytica | Hyp-Ala + NH3 | - |
ir | |
| Leu-Gly-Gly + H2O | - |
Hathewaya histolytica | Leu + Gly-Gly | - |
ir | |
| Met-Gly-Gly + H2O | - |
Hathewaya histolytica | Met + Gly-Gly | - |
ir | |
| Met-Met + H2O | - |
Hathewaya histolytica | Met + Met | - |
ir | |
| Peptides + H2O | - |
Hathewaya histolytica | ? | - |
? | |
| Phe-Gly-Gly + H2O | - |
Hathewaya histolytica | Phe + Gly-Gly | - |
ir | |
| Pro-Gly-Gly + H2O | - |
Hathewaya histolytica | Pro + Gly-Gly | - |
ir | |
| Pro-Gly-Pro + H2O | - |
Hathewaya histolytica | Pro + Gly-Pro | - |
? | |
| Pro-Gly-Pro-Pro + H2O | - |
Hathewaya histolytica | Pro + Gly-Pro-Pro | - |
ir | |
| Pro-Leu + H2O | - |
Hathewaya histolytica | Pro + Leu | - |
ir | |
| Pro-Phe + H2O | - |
Hathewaya histolytica | Pro + Phe | - |
ir | |
| Ser-Gly-Gly + H2O | - |
Hathewaya histolytica | Ser + Gly-Gly | - |
ir | |
| Val-Gly-Gly + H2O | - |
Hathewaya histolytica | Val + Gly-Gly | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 51000-59000, SDS-PAGE | Hathewaya histolytica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Hathewaya histolytica | |
| 276 | - |
Val-Gly-Gly | - |
Hathewaya histolytica | |
| 337 | - |
Pro-Gly-Pro-Pro | - |
Hathewaya histolytica | |
| 561 | - |
Pro-Gly-Pro | - |
Hathewaya histolytica | |
| 667 | - |
Phe-Gly-Gly | - |
Hathewaya histolytica | |
| 917 | - |
Leu-Gly-Gly | - |
Hathewaya histolytica | |
| 1360 | - |
Pro-Gly-Gly | - |
Hathewaya histolytica | |
| 1830 | - |
Ala-Gly-Gly | - |
Hathewaya histolytica | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
assay at | Hathewaya histolytica |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
