Literature summary for 3.4.11.10 extracted from

Kishor, N.; Guptasarma, P.
Direct N-terminal sequencing of polypeptides using a thermostable bacterial aminopeptidase and MALDI-TOF mass spectrometry (2015), Anal. Biochem., 488, 6-8 .

Search for more literature for 3.4.11.10

Application

Application	Comment	Organism
analysis	evaluation of a single-stage MS-based technique for amino acid sequencing involving partial, heterogenous digestion of a peptide by a processive, non-specific, thermotropic Bacillus subtilis-derived aminopeptidase (BsuAP), which allows single-shot sequencing to be carried out through simultaneous accumulation, and detection of subpopulations of peptides of progressively reducing length	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P25152	-	-
Bacillus subtilis 168	P25152	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	sequences and masses of peptides of different length, which are generated by the recombinant BsuAP aminopeptidase from the peptide substrate, GFP B, overview. The GFP B peptide is 14 residues long and the enzyme progressively truncates the peptide by one amino acid residue from its N-terminus. BsuAP has N-terminal de-blocking capability	Bacillus subtilis	?	-	?
additional information	sequences and masses of peptides of different length, which are generated by the recombinant BsuAP aminopeptidase from the peptide substrate, GFP B, overview. The GFP B peptide is 14 residues long and the enzyme progressively truncates the peptide by one amino acid residue from its N-terminus. BsuAP has N-terminal de-blocking capability	Bacillus subtilis 168	?	-	?

Synonyms

Synonyms	Comment	Organism
Bsu aminopeptidase	-	Bacillus subtilis
BsuAP	-	Bacillus subtilis
ywaD	-	Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
70	-	the enzyme BsuAP is not just thermostable but also thermophilic in character and activity	Bacillus subtilis

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Release 2023.1 (January 2023)