Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Streptomyces rimosus | |
amastatin | - |
Streptomyces rimosus | |
bestatin | - |
Streptomyces rimosus | |
Cu2+ | at concentrations higher than 0.1 mM | Streptomyces rimosus | |
EDTA | - |
Streptomyces rimosus | |
Mn2+ | at concentrations higher than 0.1 mM | Streptomyces rimosus | |
Ni2+ | at concentrations higher than 0.1 mM | Streptomyces rimosus | |
puromycin | - |
Streptomyces rimosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation | Streptomyces rimosus | |
Co2+ | activation | Streptomyces rimosus | |
Mg2+ | activation | Streptomyces rimosus | |
Mn2+ | activation | Streptomyces rimosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27500 | - |
gel filtration, SDS-PAGE | Streptomyces rimosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces rimosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces rimosus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Streptomyces rimosus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
156 | - |
substrate leucine-2-naphthylamide | Streptomyces rimosus |
Storage Stability | Organism |
---|---|
frozen, 6 months | Streptomyces rimosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-amino acid-peptide + H2O | best substrate with L-Leu at N-terminal end | Streptomyces rimosus | amino acid + peptide | - |
? | |
L-amino acid-peptide + H2O | influence of amino acid in penultimate position | Streptomyces rimosus | amino acid + peptide | - |
? | |
Leu-2-naphthylamide + H2O | Leu replaced by Met, Phe | Streptomyces rimosus | leucine + 2-naphthylamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 27500, SDS-PAGE | Streptomyces rimosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
40 min, no activity | Streptomyces rimosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.2 | substrate L-Leu-2-naphthylamide | Streptomyces rimosus |
8.2 | 8.55 | substrate L-Leu-4-nitroanilide | Streptomyces rimosus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | - |
Streptomyces rimosus |