Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Mycoplasma hyopneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
amastatin | - |
Mycoplasma hyopneumoniae | |
bestatin | - |
Mycoplasma hyopneumoniae | |
EDTA | - |
Mycoplasma hyopneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inhibits activity | Mycoplasma hyopneumoniae | |
Co2+ | enhances activity | Mycoplasma hyopneumoniae | |
Cu2+ | inhibits activity | Mycoplasma hyopneumoniae | |
Mg2+ | enhances activity | Mycoplasma hyopneumoniae | |
Mn2+ | enhances activity | Mycoplasma hyopneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51400 | - |
calculated from cDNA | Mycoplasma hyopneumoniae |
500000 | - |
native PAGE, rMHJ_0461 forms multimers between 500 kDa-800 kDa | Mycoplasma hyopneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mycoplasma hyopneumoniae | MHJ_0461 binds heparin and interacts with DNA | ? | - |
? | |
additional information | Mycoplasma hyopneumoniae | rMHJ_0461 binds plasminogen and facilitates plasmin conversion | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycoplasma hyopneumoniae | Q4A9M4 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Mycoplasma hyopneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-7-amido-4-methylcoumarin + H2O | enzyme shows only weak activity with this substrate | Mycoplasma hyopneumoniae | glycine + 7-amino-4-methylcoumarin | - |
? | |
L-Ala-7-amido-4-methylcoumarin + H2O | - |
Mycoplasma hyopneumoniae | L-Ala + 7-amino-4-methylcoumarin | - |
? | |
L-Arg-7-amido-4-methylcoumarin + H2O | - |
Mycoplasma hyopneumoniae | L-Arg + 7-amino-4-methylcoumarin | - |
? | |
L-Ile-7-amido-4-methylcoumarin + H2O | enzyme shows only weak activity with this substrate | Mycoplasma hyopneumoniae | L-isoleucine + 7-amino-4-methylcoumarin | - |
? | |
L-Leu-7-amido-4-methylcoumarin + H2O | - |
Mycoplasma hyopneumoniae | L-Leu + 7-amino-4-methylcoumarin | - |
? | |
L-Met-7-amido-4-methylcoumarin + H2O | - |
Mycoplasma hyopneumoniae | L-Met + 7-amino-4-methylcoumarin | - |
? | |
L-Phe-7-amido-4-methylcoumarin + H2O | - |
Mycoplasma hyopneumoniae | L-Phe + 7-amino-4-methylcoumarin | - |
? | |
L-Val-7-amido-4-methylcoumarin + H2O | enzyme shows only weak activity with this substrate | Mycoplasma hyopneumoniae | L-Val + 7-amino-4-methylcoumarin | - |
? | |
additional information | MHJ_0461 binds heparin and interacts with DNA | Mycoplasma hyopneumoniae | ? | - |
? | |
additional information | rMHJ_0461 binds plasminogen and facilitates plasmin conversion | Mycoplasma hyopneumoniae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | - |
Mycoplasma hyopneumoniae |
Synonyms | Comment | Organism |
---|---|---|
rMHJ_0461 | - |
Mycoplasma hyopneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycoplasma hyopneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
- |
Mycoplasma hyopneumoniae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 10 | - |
Mycoplasma hyopneumoniae |
General Information | Comment | Organism |
---|---|---|
physiological function | rMHJ_0461 binds plasminogen and facilitates plasmin conversion | Mycoplasma hyopneumoniae |