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Literature summary for 3.4.11.1 extracted from

  • Slutskaya, E.S.; Bezsudnova, E.Y.; Mardanov, A.V.; Gumerov, V.M.; Rakitina, T.V.; Popov, V.O.; Lipkin, V.M.
    Characterization of a novel M42 aminopeptidase from crenarchaeon Desulfurococcus kamchatkensis (2012), Dokl. Biochem. Biophys., 442, 30-32.
    View publication on PubMed
Search for more literature for 3.4.11.1

Inhibitors

Inhibitors Comment Organism Structure
bestatin
-
 Desulfurococcus amylolyticus
EDTA
-
 Desulfurococcus amylolyticus
additional information leupeptin, aprotinin, and pepstatin virtually have no effect on the enzyme activity Desulfurococcus amylolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 L-Leu-4-nitroanilide pH 7.5, 50°C, enzyme activated by heating at 70°C Desulfurococcus amylolyticus
1.8
-
 L-Leu-4-nitroanilide pH 7.5, 50°C Desulfurococcus amylolyticus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ maximum activity of the apoenzyme is observed in the presence of Mg2+, Mn2+, and Zn2+ Desulfurococcus amylolyticus
Mn2+ maximum activity of the apoenzyme is observed in the presence of Mg2+, Mn2+, and Zn2+ Desulfurococcus amylolyticus
Zn2+ maximum activity of the apoenzyme is observed in the presence of Mg2+, Mn2+, and Zn2+ Desulfurococcus amylolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43200
-
 2 * 43200, octamer/dimer mixture at the ratio 2:1, SDS-PAGE Desulfurococcus amylolyticus
43200
-
 8 * 43200, octamer/dimer mixture at the ratio 2:1, SDS-PAGE Desulfurococcus amylolyticus

Organism

Organism UniProt Comment Textmining
Desulfurococcus amylolyticus B8D484
-
-
Desulfurococcus amylolyticus DSM 18924 B8D484
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Desulfurococcus amylolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1
-
 pH 7.5, 50°C, substrate: L-Leu-4-nitroanilide Desulfurococcus amylolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu-4-nitroanilide + H2O
-
 Desulfurococcus amylolyticus L-Leu + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O
-
 Desulfurococcus amylolyticus DSM 18924 L-Leu + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 43200, octamer/dimer mixture at the ratio 2:1, SDS-PAGE Desulfurococcus amylolyticus
octamer 8 * 43200, octamer/dimer mixture at the ratio 2:1, SDS-PAGE Desulfurococcus amylolyticus

Synonyms

Synonyms Comment Organism
APDkam589
-
 Desulfurococcus amylolyticus
M42 aminopeptidase
-
 Desulfurococcus amylolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Desulfurococcus amylolyticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 heating APDkam589 (600 µg/ml) at 70°C for 1 h lead to an increase in the specific activity to 20-22 U/mg. Further heating of the enzyme preparation does not lead to an increase of the activity. After cooling to room temperature the resulting enzyme activity is retained within 12 h. Apparently, the heating of the protein switches it to a more active conformation Desulfurococcus amylolyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4
-
 L-Leu-4-nitroanilide pH 7.5, 50°C Desulfurococcus amylolyticus
19
-
 L-Leu-4-nitroanilide pH 7.5, 50°C, enzyme activated by heating at 70°C Desulfurococcus amylolyticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3
-
 L-Leu-4-nitroanilide pH 7.5, 50°C Desulfurococcus amylolyticus
15.8
-
 L-Leu-4-nitroanilide pH 7.5, 50°C, enzyme activated by heating at 70°C Desulfurococcus amylolyticus