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Literature summary for 3.4.11.1 extracted from

  • Kang, J.; Ju, H.; Sohn, W.; Na, B.
    Molecular cloning and characterization of a M17 leucine aminopeptidase of Cryptosporidium parvum (2011), Parasitology, 138, 682-690.
    View publication on PubMed
Search for more literature for 3.4.11.1

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged protein expressed in Escherichia coli M15 [pREP4] Cryptosporidium parvum

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 7.2% residual activity at 1 mM Cryptosporidium parvum
bestatin 0.4% residual activity at 0.001 mM Cryptosporidium parvum
EDTA 6.7% residual activity at 1 mM Cryptosporidium parvum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.102
-
 L-leucine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
0.1092
-
 L-methionine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
0.2396
-
 L-alanine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
0.245
-
 L-phenylalanine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
0.3641
-
 L-lysine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 47.8fold activity increase at 1 mM Cryptosporidium parvum
Mg2+ 10.9fold activity increase at 1 mM Cryptosporidium parvum
Mn2+ 64.3fold activity increase at 1 mM Cryptosporidium parvum
Zn2+ 12.1fold activity increase at 1 mM Cryptosporidium parvum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 x * 58000, SDS-PAGE Cryptosporidium parvum

Organism

Organism UniProt Comment Textmining
Cryptosporidium parvum Q5CRI9
-
-
Cryptosporidium parvum Iowa II Q5CRI9
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+) Cryptosporidium parvum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine-4-methylcoumaryl-7-amide + H2O
-
 Cryptosporidium parvum L-alanine + 7-amino-4-methylcoumarin
-
 ?
L-alanine-4-methylcoumaryl-7-amide + H2O
-
 Cryptosporidium parvum Iowa II L-alanine + 7-amino-4-methylcoumarin
-
 ?
L-leucine-4-methylcoumaryl-7-amide + H2O
-
 Cryptosporidium parvum L-leucine + 7-amino-4-methylcoumarin
-
 ?
L-leucine-4-methylcoumaryl-7-amide + H2O
-
 Cryptosporidium parvum Iowa II L-leucine + 7-amino-4-methylcoumarin
-
 ?
L-lysine-4-methylcoumaryl-7-amide + H2O
-
 Cryptosporidium parvum L-lysine + 7-amino-4-methylcoumarin
-
 ?
L-lysine-4-methylcoumaryl-7-amide + H2O
-
 Cryptosporidium parvum Iowa II L-lysine + 7-amino-4-methylcoumarin
-
 ?
L-methionine-4-methylcoumaryl-7-amide + H2O
-
 Cryptosporidium parvum L-methionine + 7-amino-4-methylcoumarin
-
 ?
L-methionine-4-methylcoumaryl-7-amide + H2O
-
 Cryptosporidium parvum Iowa II L-methionine + 7-amino-4-methylcoumarin
-
 ?
L-phenylalanine-4-methylcoumaryl-7-amide + H2O
-
 Cryptosporidium parvum L-phenylalanine + 7-amino-4-methylcoumarin
-
 ?
L-phenylalanine-4-methylcoumaryl-7-amide + H2O
-
 Cryptosporidium parvum Iowa II L-phenylalanine + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 58000, SDS-PAGE Cryptosporidium parvum

Synonyms

Synonyms Comment Organism
M17 leucine aminopeptidase
-
 Cryptosporidium parvum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
 L-lysine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
0.01
-
 L-alanine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
0.075
-
 L-phenylalanine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
0.085
-
 L-methionine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
0.107
-
 L-leucine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 broad pH optimal range: pH 7.5-9.5 Cryptosporidium parvum

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
 highly unstable in acidic pHs, but relatively stable at neutral and alkaline conditions Cryptosporidium parvum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0054
-
 L-lysine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
0.0417
-
 L-alanine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
0.3061
-
 L-phenylalanine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
0.7784
-
 L-methionine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum
1.049
-
 L-leucine-4-methylcoumaryl-7-amide pH 8.5, 37°C Cryptosporidium parvum