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Literature summary for 3.4.11.1 extracted from

  • Cadavid-Restrepo, G.; Gastardelo, T.; Faudry, E.; De Almeida, H.; Bastos, I.; Negreiros, R.; Lima, M.; Assump?ß?£o, T.; Almeida, K.; Ragno, M.; Ebel, C.; Ribeiro, B.; Felix, C.; Santana, J.
    The major leucyl aminopeptidase of Trypanosoma cruzi (LAPTc) assembles into a homohexamer and belongs to the M17 family of metallopeptidases (2011), BMC Biochem., 12, 46.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.1

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged protein expressed in Escherichia coli BL21(DE3) Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 0.25 mM: 17% activity, restored by Mn2+ and Ca2+ Trypanosoma cruzi
bestatin 0.1 mM: 3% activity, activity Trypanosoma cruzi
EDTA 10 mM: 55% activity, restored by Mn2+ and Ca2+ Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 L-Leu-7-amido-4-methylcoumarin native enzyme, pH 7.5, 37°C Trypanosoma cruzi
0.1859
-
 L-Leu-7-amido-4-methylcoumarin recombinant enzyme, pH 7.5, 37°C Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 6 * 55000, SDS-PAGE, calculated: 55891 Da Trypanosoma cruzi
320000
-
 gel filtration Trypanosoma cruzi
330000
-
 analytical ultracentrifugation Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi Q4DZJ3
-
-

Purification (Commentary)

Purification (Comment) Organism
ion exchange chromatography (DEAE), gel filtration; His-tagged protein: immobilized metal ion affinity chromatography (Ni2+), gel filtration Trypanosoma cruzi

Source Tissue

Source Tissue Comment Organism Textmining
epimastigote
-
 Trypanosoma cruzi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma cruzi L-Leu + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
homohexamer 6 * 55000, SDS-PAGE, calculated: 55891 Da Trypanosoma cruzi

Synonyms

Synonyms Comment Organism
major leucyl aminopeptidase metalloaminopeptidase Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 native enzyme Trypanosoma cruzi
60
-
 recombinant enzyme, 25% activity at 37°C Trypanosoma cruzi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 recombinant enzyme is more stable than native enzyme: after 15 min at 60°C native form has 35% and recombinant form 87% activity Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.47
-
 L-Leu-7-amido-4-methylcoumarin native enzyme, pH 7.5, 37°C Trypanosoma cruzi
34.84
-
 L-Leu-7-amido-4-methylcoumarin recombinant enzyme, pH 7.5, 37°C Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 native enzyme Trypanosoma cruzi
8
-
 recombinant enzyme Trypanosoma cruzi

pH Range

pH Minimum pH Maximum Comment Organism
6 8 native enzyme, 45% activity at pH 6.0 and 8.0, inactive at pH 5.0 and 9.0 Trypanosoma cruzi
7.5 9 recombinant enzyme, 40% activity at pH 7.5, 75% activity at pH 9.0 Trypanosoma cruzi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
190
-
 L-Leu-7-amido-4-methylcoumarin recombinant enzyme, pH 7.5, 37°C Trypanosoma cruzi
1040
-
 L-Leu-7-amido-4-methylcoumarin native enzyme, pH 7.5, 37°C Trypanosoma cruzi