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Literature summary for 3.4.11.1 extracted from

  • Vujcic, Z.; Loncar, N.; Dojnov, B.; Milovanovic, A.; Vujcic, M.; Bozic, N.
    Characterisation of leucyl aminopeptidase from Solanum tuberosum tuber (2010), Food Chem., 121, 418-423.
    View publication on PubMed
Search for more literature for 3.4.11.1

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 75% inhibition at 2 mM Solanum tuberosum
cysteine 20% inhibition at 2 mM Solanum tuberosum
additional information no or poor inhibition by 2 mM of iodoacetic acid, EDTA, EGTA, and citrate Solanum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00925
-
 L-leucyl-4-nitroanilide pH 9.0, 37°C Solanum tuberosum
0.083
-
 L-methionyl-4-nitroanilide pH 9.0, 37°C Solanum tuberosum
0.099
-
 L-Lysyl-4-nitroanilide pH 9.0, 37°C Solanum tuberosum
0.36
-
 L-alanyl-4-nitroanilide pH 9.0, 37°C Solanum tuberosum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ stimulates by 100% Solanum tuberosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 2 * 48000, SDS-PAGE Solanum tuberosum
90000
-
 gel filtration Solanum tuberosum

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
 cv. Kennebec
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 200fold from tubers by gel filtration, anion exchange chromatography, and again gel filtration to homogeneity Solanum tuberosum

Source Tissue

Source Tissue Comment Organism Textmining
tuber 1-year-old tubers Solanum tuberosum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanyl-4-nitroanilide + H2O
-
 Solanum tuberosum L-alanine + 4-nitroaniline
-
 ?
L-leucyl-4-nitroanilide + H2O
-
 Solanum tuberosum L-leucine + 4-nitroaniline
-
 ?
L-lysyl-4-nitroanilide + H2O
-
 Solanum tuberosum L-lysine + 4-nitroaniline
-
 ?
L-methionyl-4-nitroanilide + H2O
-
 Solanum tuberosum L-methionine + 4-nitroaniline
-
 ?
additional information the enzyme is equally active with the synthetic substrates Solanum tuberosum ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 48000, SDS-PAGE Solanum tuberosum

Synonyms

Synonyms Comment Organism
LAP
-
 Solanum tuberosum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Solanum tuberosum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 activity range, profile, overview Solanum tuberosum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 purified enzyme, inactivation Solanum tuberosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Solanum tuberosum

pH Range

pH Minimum pH Maximum Comment Organism
5 10.5 activity range, profile, overview Solanum tuberosum

pI Value

Organism Comment pI Value Maximum pI Value
Solanum tuberosum isoelectric focusing
-
 5.45