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Literature summary for 3.4.11.1 extracted from

  • McCarthy, E.; Stack, C.; Donnelly, S.M.; Doyle, S.; Mann, V.H.; Brindley, P.J.; Stewart, M.; Day, T.A.; Maule, A.G.; Dalton, J.P.
    Leucine aminopeptidase of the human blood flukes, Schistosoma mansoni and Schistosoma japonicum (2004), Int. J. Parasitol., 34, 703-714.
    View publication on PubMed
Search for more literature for 3.4.11.1

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression of His6-tagged enzyme in Spodoptera frugiperda Sf9 insect cells using the baculovirus transfection system Schistosoma mansoni

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 61.5% inhibition at 5 mM Schistosoma japonicum
1,10-phenanthroline 71% inhibition at 5 mM Schistosoma mansoni
bestatin nearly complete inhibition at 0.05 mM Schistosoma japonicum
bestatin nearly complete inhibition at 0.05 mM Schistosoma mansoni
EDTA 50% inhibition at 5 mM Schistosoma mansoni
additional information no inhibition by EDTA Schistosoma japonicum

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Schistosoma mansoni
-
-
soluble
-
 Schistosoma japonicum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ about 2fold activation at 0.5 mM for the recombinant enzyme and about 4fold for the wild-type enzyme in cell extract, metallopeptidase Schistosoma mansoni
Mg2+ about 3.5fold activation at 0.5 mM, metallopeptidase Schistosoma japonicum
Mn2+ about 2fold activation at 0.05 mM for the recombinant enzyme and about 4fold for the wild-type enzyme in cell extract, metallopeptidase Schistosoma mansoni
Mn2+ about 4fold activation at 0.05 mM, metallopeptidase Schistosoma japonicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57500
-
 x * 57500, recombinant enzyme, SDS-PAGE Schistosoma mansoni

Organism

Organism UniProt Comment Textmining
Schistosoma japonicum Q9GQ37 incomplete sequence missing 26 N-terminal amino acids
-
Schistosoma mansoni
-
 synthetic construct
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Spodoptera frugiperda Sf9 insect cells by nickel affinity chromatography Schistosoma mansoni

Reaction

Reaction Comment Organism Reaction ID
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid mides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low endopeptidase Schistosoma mansoni
a
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid mides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low endopeptidase Schistosoma japonicum
a

Source Tissue

Source Tissue Comment Organism Textmining
gut gastrodermal cells surrounding the gut lumen Schistosoma mansoni
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Schistosoma mansoni
additional information
-
-
 Schistosoma japonicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanyl-7-amido-4-methylcoumarin + H2O
-
 Schistosoma mansoni L-alanine + 7-amino-4-methylcoumarin
-
 ?
L-alanyl-7-amido-4-methylcoumarin + H2O
-
 Schistosoma japonicum L-alanine + 7-amino-4-methylcoumarin
-
 ?
L-leucyl-7-amido-4-methylcoumarin + H2O best substrate Schistosoma mansoni L-leucine + 7-amino-4-methylcoumarin
-
 ?
L-leucyl-7-amido-4-methylcoumarin + H2O best substrate Schistosoma japonicum L-leucine + 7-amino-4-methylcoumarin
-
 ?
L-prolyl-7-amido-4-methylcoumarin + H2O
-
 Schistosoma mansoni L-proline + 7-amino-4-methylcoumarin
-
 ?
L-prolyl-7-amido-4-methylcoumarin + H2O
-
 Schistosoma japonicum L-proline + 7-amino-4-methylcoumarin
-
 ?
L-tyrosyl-7-amido-4-methylcoumarin + H2O
-
 Schistosoma mansoni L-tyrosine + 7-amino-4-methylcoumarin
-
 ?
L-tyrosyl-7-amido-4-methylcoumarin + H2O
-
 Schistosoma japonicum L-tyrosine + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 57500, recombinant enzyme, SDS-PAGE Schistosoma mansoni

Synonyms

Synonyms Comment Organism
LAP
-
 Schistosoma mansoni
LAP
-
 Schistosoma japonicum
leucine aminopeptidase
-
 Schistosoma mansoni
leucine aminopeptidase
-
 Schistosoma japonicum
More the enzyme belongs to the M17 leucine peptidase family Schistosoma mansoni
More the enzyme belongs to the M17 leucine peptidase family Schistosoma japonicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Schistosoma mansoni
37
-
 assay at Schistosoma japonicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.25
-
-
 Schistosoma mansoni
8.25
-
-
 Schistosoma japonicum

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.4 no activity below pH 6.5 Schistosoma mansoni