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Literature summary for 3.4.11.1 extracted from

  • Yamazaki, T.; Akada, T.; Niizeki, O.; Suzuki, T.; Miyashita, H.; Sato, Y.
    Puromycin-insensitive leucyl-specific aminopeptidase (PILSAP) binds and catalyzes PDK1, allowing VEGF-stimulated activation of S6K for endothelial cell proliferation and angiogenesis (2004), Blood, 104, 2345-2352.
    View publication on PubMed
Search for more literature for 3.4.11.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), transient expression in COS-7 cells and in HEK-293 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
E343A site-directed mutagenesis, inactive, dominant-negative mutant Mus musculus
E365A site-directed mutagenesis, inactive mutant Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
additional information vascular endothelial growth factor-stimulated G1/S transition in endothelial cells inhibits the enzyme activity and/or expression leading to an arrest in G1 phase Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ metallopeptidase, contains the conserved motif HEXXH(18X)E Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus the enzyme is required for DNA synthesis and plays an important role in angiogenesis by regulating the proliferation and migration of endothelial cells, mechanism via vascular endothelial growth factor and stimulation of S6 kinase, overview ?
-
 ?
phosphatidylinositol-dependent kinase-1 + H2O Mus musculus i.e. PDK1, removal of 9 amino acids from the N-terminus of the kinase, which allows S6 kinase to associate with PDK1 and the enzyme upon vascular endothelial growth factor stimulation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme partially from COS-7 cells Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
MSS31 cell
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-leucyl-7-amido-4-methylcoumarin + H2O
-
 Mus musculus L-leucine + 7-amino-4-methylcoumarin
-
 ?
additional information the enzyme is required for DNA synthesis and plays an important role in angiogenesis by regulating the proliferation and migration of endothelial cells, mechanism via vascular endothelial growth factor and stimulation of S6 kinase, overview Mus musculus ?
-
 ?
phosphatidylinositol-dependent kinase-1 + H2O i.e. PDK1, removal of 9 amino acids from the N-terminus of the kinase, which allows S6 kinase to associate with PDK1 and the enzyme upon vascular endothelial growth factor stimulation Mus musculus ?
-
 ?
phosphatidylinositol-dependent kinase-1 + H2O i.e. PDK1, removal of 9 amino acids from the N-terminus of the kinase Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
PILSAP
-
 Mus musculus
puromycin-insensitive leucyl-specific aminopeptidase
-
 Mus musculus