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Literature summary for 3.3.2.9 extracted from

  • Kim, H.S.; Lee, S.J.; Lee, E.J.; Hwang, J.W.; Park, S.; Kim, S.J.; Lee, E.Y.
    Cloning and characterization of a fish microsomal epoxide hydrolase of Danio rerio and application to kinetic resolution of racemic styrene oxide (2005), J. Mol. Catal. B, 37, 30-35.
No PubMed abstract available
Search for more literature for 3.3.2.9

Application

Application Comment Organism
synthesis the purified recombinant enzyme can be used as biocatalyst for kinetic resolution of racemic styrene oxide with the result of over 99% enantiopure (S)-styrene oxide in 23,5% yield Danio rerio

Cloned(Commentary)

Cloned (Comment) Organism
functional expression in Escherichia coli Danio rerio

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Danio rerio
-
-

Organism

Organism UniProt Comment Textmining
Danio rerio
-
-
-

Reaction

Reaction Comment Organism Reaction ID
cis-stilbene oxide + H2O = (1R,2R)-1,2-diphenylethane-1,2-diol the catalytic triad is Asp223-Glu402-His429, the active site includes an oxyanion hole Danio rerio
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.4
-
-
 Danio rerio

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-stilbene oxide + H2O the recombinant enzyme expressed in Escherichia coli acts stereospecifically on the the (R)-styrene Danio rerio (+)-(1R,2R)-1,2-diphenylethane-1,2-diol
-
 ?

Synonyms

Synonyms Comment Organism
mEH
-
 Danio rerio
mEH-like protein
-
 Danio rerio
microsomal epoxide hydrolase-like protein
-
 Danio rerio