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Literature summary for 3.3.2.9 extracted from

  • Wixtrom, R.N.; Hammock, B.D.
    Membrane-bound and soluble-fraction epoxide hydrolases (1985), Biochem. Pharmacol. Toxicol., 1, 1-93.
No PubMed abstract available
Search for more literature for 3.3.2.9

Activating Compound

Activating Compound Comment Organism Structure
additional information induction of the enzyme by clofibrate and di(2-ethylhexyl)phthalate Mus musculus
additional information induction of the enzyme by clofibrate and di(2-ethylhexyl)phthalate Homo sapiens
additional information induction of the enzyme by clofibrate and di(2-ethylhexyl)phthalate Rattus norvegicus
additional information induction of the enzyme by clofibrate and di(2-ethylhexyl)phthalate Oryctolagus cuniculus
additional information induction of the enzyme by clofibrate and di(2-ethylhexyl)phthalate Macaca mulatta

Inhibitors

Inhibitors Comment Organism Structure
1,1,1-Trichloropropene 2,3-oxide
-
 Homo sapiens
1,1,1-Trichloropropene 2,3-oxide
-
 Macaca mulatta
1,1,1-Trichloropropene 2,3-oxide
-
 Mus musculus
1,1,1-Trichloropropene 2,3-oxide
-
 Oryctolagus cuniculus
1,1,1-Trichloropropene 2,3-oxide
-
 Rattus norvegicus
2-Bromo-4'-nitroacetophenone
-
 Homo sapiens
2-Bromo-4'-nitroacetophenone
-
 Macaca mulatta
2-Bromo-4'-nitroacetophenone
-
 Mus musculus
2-Bromo-4'-nitroacetophenone
-
 Oryctolagus cuniculus
2-Bromo-4'-nitroacetophenone
-
 Rattus norvegicus
Cu2+
-
 Homo sapiens
Cu2+
-
 Macaca mulatta
Cu2+
-
 Mus musculus
Cu2+
-
 Oryctolagus cuniculus
Cu2+
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Mus musculus
-
-
soluble
-
 Homo sapiens
-
-
soluble
-
 Rattus norvegicus
-
-
soluble
-
 Oryctolagus cuniculus
-
-
soluble
-
 Macaca mulatta
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Macaca mulatta
-
-
-
Mus musculus
-
-
-
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially 2.4fold from liver using PEG precipitation, and to homogeneity by ion exchange, hydrophobic interaction chromatography, and hydroxylapatite chromatography Mus musculus
partially from liver Homo sapiens
partially from liver Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
kidney high activity Mus musculus
-
kidney high activity Homo sapiens
-
kidney high activity Rattus norvegicus
-
kidney high activity Oryctolagus cuniculus
-
kidney high activity Macaca mulatta
-
liver high activity Mus musculus
-
liver high activity Homo sapiens
-
liver high activity Rattus norvegicus
-
liver high activity Oryctolagus cuniculus
-
liver high activity Macaca mulatta
-
testis low activity Mus musculus
-
testis low activity Homo sapiens
-
testis low activity Rattus norvegicus
-
testis low activity Oryctolagus cuniculus
-
testis low activity Macaca mulatta
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 several analytical and activity assay methods Mus musculus
additional information
-
 several analytical and activity assay methods Homo sapiens
additional information
-
 several analytical and activity assay methods Rattus norvegicus
additional information
-
 several analytical and activity assay methods Oryctolagus cuniculus
additional information
-
 several analytical and activity assay methods Macaca mulatta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-stilbene oxide + H2O
-
 Mus musculus (+)-(1R,2R)-1,2-diphenylethane-1,2-diol
-
 ?
cis-stilbene oxide + H2O
-
 Homo sapiens (+)-(1R,2R)-1,2-diphenylethane-1,2-diol
-
 ?
cis-stilbene oxide + H2O
-
 Rattus norvegicus (+)-(1R,2R)-1,2-diphenylethane-1,2-diol
-
 ?
cis-stilbene oxide + H2O
-
 Oryctolagus cuniculus (+)-(1R,2R)-1,2-diphenylethane-1,2-diol
-
 ?
cis-stilbene oxide + H2O
-
 Macaca mulatta (+)-(1R,2R)-1,2-diphenylethane-1,2-diol
-
 ?
clofibrate + H2O
-
 Mus musculus ?
-
 ?
clofibrate + H2O
-
 Homo sapiens ?
-
 ?
clofibrate + H2O
-
 Rattus norvegicus ?
-
 ?
clofibrate + H2O
-
 Oryctolagus cuniculus ?
-
 ?
clofibrate + H2O
-
 Macaca mulatta ?
-
 ?
di(2-ethylhexyl)phthalate + H2O
-
 Mus musculus ?
-
 ?
di(2-ethylhexyl)phthalate + H2O
-
 Homo sapiens ?
-
 ?
di(2-ethylhexyl)phthalate + H2O
-
 Rattus norvegicus ?
-
 ?
di(2-ethylhexyl)phthalate + H2O
-
 Oryctolagus cuniculus ?
-
 ?
di(2-ethylhexyl)phthalate + H2O
-
 Macaca mulatta ?
-
 ?
additional information substrate specificity, no activity with 1,2,3,4,9,9-hexachloro-6,7-epoxy-1,4,41,5,6,7,8,8a-octahydro-1,4-methanonaphthalene,i.e. HEOM, 2-acetylaminofluorene, arochlor 1254, and benzopyrene 4,5-oxide, the cytosolic enzyme hydrates epoxides on cyclic systems very slowly, but hydrates a wide variety of aliphatic epoxides rapidly, trisubstituted terpenoid epoxides show low Km-valus, fatty acid and ester epoxides are hydrated very quickly Macaca mulatta ?
-
 ?
additional information substrate specificity, no activity with 1,2,3,4,9,9-hexachloro-6,7-epoxy-1,4,41,5,6,7,8,8a-octahydro-1,4-methanonaphthalene,i.e. HEOM, 2-acetylaminofluorene, arochlor 1254, and benzopyrene 4,5-oxide, the cytosolic enzyme hydrates epoxides on cyclic sytems very slowly, but hydrates a wide variety of aliphatic epoxides rapidly, trisubstituted terpenoid epoxides show low Km-values, fatty acid and ester epoxides are hydrated very quickly Mus musculus ?
-
 ?
additional information substrate specificity, no activity with 1,2,3,4,9,9-hexachloro-6,7-epoxy-1,4,41,5,6,7,8,8a-octahydro-1,4-methanonaphthalene,i.e. HEOM, 2-acetylaminofluorene, arochlor 1254, and benzopyrene 4,5-oxide, the cytosolic enzyme hydrates epoxides on cyclic sytems very slowly, but hydrates a wide variety of aliphatic epoxides rapidly, trisubstituted terpenoid epoxides show low Km-values, fatty acid and ester epoxides are hydrated very quickly Homo sapiens ?
-
 ?
additional information substrate specificity, no activity with 1,2,3,4,9,9-hexachloro-6,7-epoxy-1,4,41,5,6,7,8,8a-octahydro-1,4-methanonaphthalene,i.e. HEOM, 2-acetylaminofluorene, arochlor 1254, and benzopyrene 4,5-oxide, the cytosolic enzyme hydrates epoxides on cyclic sytems very slowly, but hydrates a wide variety of aliphatic epoxides rapidly, trisubstituted terpenoid epoxides show low Km-values, fatty acid and ester epoxides are hydrated very quickly Rattus norvegicus ?
-
 ?
additional information substrate specificity, no activity with 1,2,3,4,9,9-hexachloro-6,7-epoxy-1,4,41,5,6,7,8,8a-octahydro-1,4-methanonaphthalene,i.e. HEOM, 2-acetylaminofluorene, arochlor 1254, and benzopyrene 4,5-oxide, the cytosolic enzyme hydrates epoxides on cyclic sytems very slowly, but hydrates a wide variety of aliphatic epoxides rapidly, trisubstituted terpenoid epoxides show low Km-values, fatty acid and ester epoxides are hydrated very quickly Oryctolagus cuniculus ?
-
 ?
styrene oxide + H2O
-
 Mus musculus 1-phenylethane-1,2-diol
-
 ?
styrene oxide + H2O
-
 Homo sapiens 1-phenyl-ethane-1,2-diol
-
 ?
styrene oxide + H2O
-
 Rattus norvegicus 1-phenyl-ethane-1,2-diol
-
 ?
styrene oxide + H2O
-
 Oryctolagus cuniculus 1-phenyl-ethane-1,2-diol
-
 ?
styrene oxide + H2O
-
 Macaca mulatta 1-phenyl-ethane-1,2-diol
-
 ?
trans-9,10-epoxystearate + H2O
-
 Mus musculus 9,10-dihydroxy-octadecanoic acid
-
 ?
trans-9,10-epoxystearate + H2O
-
 Homo sapiens 9,10-dihydroxy-octadecanoic acid
-
 ?
trans-9,10-epoxystearate + H2O
-
 Rattus norvegicus 9,10-dihydroxy-octadecanoic acid
-
 ?
trans-9,10-epoxystearate + H2O
-
 Oryctolagus cuniculus 9,10-dihydroxy-octadecanoic acid
-
 ?
trans-9,10-epoxystearate + H2O
-
 Macaca mulatta 9,10-dihydroxy-octadecanoic acid
-
 ?