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Literature summary for 3.3.2.2 extracted from

  • Jurkowitz, M.S.; Patel, A.; Wu, L.C.; Krautwater, A.; Pfeiffer, D.R.; Bell, C.E.
    The YhhN protein of Legionella pneumophila is a lysoplasmalogenase (2015), Biochim. Biophys. Acta, 1848, 742-751.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.3.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C43(DE3) cells as C-terminal-GFP-His8-fusion Legionella pneumophila

General Stability

General Stability Organism
both un-tagged YhhN protein and the YhhN-GFP fusion require the continuous presence of 0.1% DDM (w/v) or 2-4 mM diradylglycerophospholipid for maintenance of activity at 4°C. For storage at -20°C, the presence of 50% (v/v) glycerol or 2-4 mM diradylglycerophospholipid is also required Legionella pneumophila

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by EDTA, lysophosphatidic acid, sodium fluoride, phosphatidic acid, monoacylglycerophospho-choline, monoacylglycerophospho-ethanolamine, and phenylmethylsulfonyl fluoride Legionella pneumophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
 ethanolamine lysoplasmalogen at pH 7.1, temperature not specified in the publication Legionella pneumophila
0.045
-
 choline lysoplasmalogen at pH 7.1, temperature not specified in the publication Legionella pneumophila

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 75% residual activity at 10 mM Legionella pneumophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 x * 18000, SDS-PAGE Legionella pneumophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
choline lysoplasmalogen + H2O Legionella pneumophila
-
 ? + glycerophospho-choline
-
 ?
choline lysoplasmalogen + H2O Legionella pneumophila ATCC 33152D-5 Philadelphia-1
-
 ? + glycerophospho-choline
-
 ?
ethanolamine lysoplasmalogen + H2O Legionella pneumophila the enzyme catalyzes hydrolysis of the vinyl ether linkage at sn-1, is highly specific for the sn-2-deacylated (lyso) form of plasmalogen, and has no activity on the diradyl plasmalogens ? + glycerophospho-ethanolamine
-
 ?
ethanolamine lysoplasmalogen + H2O Legionella pneumophila ATCC 33152D-5 Philadelphia-1 the enzyme catalyzes hydrolysis of the vinyl ether linkage at sn-1, is highly specific for the sn-2-deacylated (lyso) form of plasmalogen, and has no activity on the diradyl plasmalogens ? + glycerophospho-ethanolamine
-
 ?

Organism

Organism UniProt Comment Textmining
Legionella pneumophila Q5ZU17
-
-
Legionella pneumophila ATCC 33152D-5 Philadelphia-1 Q5ZU17
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Legionella pneumophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.055
-
 cell lysate, at pH 7.1, temperature not specified in the publication Legionella pneumophila
8.443
-
 after 153.5fold purification, at pH 7.1, temperature not specified in the publication Legionella pneumophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
choline lysoplasmalogen + H2O
-
 Legionella pneumophila ? + glycerophospho-choline
-
 ?
choline lysoplasmalogen + H2O
-
 Legionella pneumophila ATCC 33152D-5 Philadelphia-1 ? + glycerophospho-choline
-
 ?
ethanolamine lysoplasmalogen + H2O the enzyme catalyzes hydrolysis of the vinyl ether linkage at sn-1, is highly specific for the sn-2-deacylated (lyso) form of plasmalogen, and has no activity on the diradyl plasmalogens Legionella pneumophila ? + glycerophospho-ethanolamine
-
 ?
ethanolamine lysoplasmalogen + H2O the enzyme catalyzes hydrolysis of the vinyl ether linkage at sn-1, is highly specific for the sn-2-deacylated (lyso) form of plasmalogen, and has no activity on the diradyl plasmalogens Legionella pneumophila ATCC 33152D-5 Philadelphia-1 ? + glycerophospho-ethanolamine
-
 ?
additional information the enzyme has no activity on diradyl plasmalogen, 1-alkenyl-glycerol, and monoacylglycerophospho-ethanolamine, ethanolamine plasmalogen, choline plasmalogen, monoacylglycerophospho-choline, lysophosphatidic acid, or monoacylglycerophospho-choline Legionella pneumophila ?
-
 ?
additional information the enzyme has no activity on diradyl plasmalogen, 1-alkenyl-glycerol, and monoacylglycerophospho-ethanolamine, ethanolamine plasmalogen, choline plasmalogen, monoacylglycerophospho-choline, lysophosphatidic acid, or monoacylglycerophospho-choline Legionella pneumophila ATCC 33152D-5 Philadelphia-1 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 18000, SDS-PAGE Legionella pneumophila

Synonyms

Synonyms Comment Organism
lysoplasmalogenase
-
 Legionella pneumophila
YhhN
-
 Legionella pneumophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
 heating the enzyme to 95°C for 3 min results in complete loss of activity Legionella pneumophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Legionella pneumophila

General Information

General Information Comment Organism
physiological function the enzyme protects the bacterium from lysis by lysoplasmalogen derived from plasmalogens of the host Legionella pneumophila