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Literature summary for 3.3.2.2 extracted from

  • Wu, L.C.; Pfeiffer, D.R.; Calhoon, E.A.; Madiai, F.; Marcucci, G.; Liu, S.; Jurkowitz, M.S.
    Purification, identification, and cloning of lysoplasmalogenase, the enzyme that catalyzes hydrolysis of the vinyl ether bond of lysoplasmalogen (2011), J. Biol. Chem., 286, 24916-24930.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.3.2.2

Cloned(Commentary)

Cloned (Comment) Organism
functional expression both in Escherichia coli and HEK 293T cell Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
lysophosphatidic acid competitive Rattus norvegicus
additional information not inhibitory at 5 mM: Ca2+, Mg2+, Mn2+, EDTA, NaF or PMSF, phosphatidic acid, sphingosine 1-phosphate, monoacylglycerophosphocholine, and monoacylglycerophosphoethanolamine Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
 lysoplasmenylcholine pH 7.1, temperature not specified in the publication. Coupled enzyme assay, the aldehyde that is produced by hydrolysis of lysoplasmalogen is reduced to an alcohol in a second enzymic reaction. In the second reaction, NADH is oxidized in a 1:1 stoichiometry between the mol of aldehyde produced and mol of NADH oxidized Rattus norvegicus
0.053
-
 lysoplasmenylethanolamine pH 7.1, temperature not specified in the publication. Coupled enzyme assay, the aldehyde that is produced by hydrolysis of lysoplasmalogen is reduced to an alcohol in a second enzymic reaction. In the second reaction, NADH is oxidized in a 1:1 stoichiometry between the mol of aldehyde produced and mol of NADH oxidized Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 x * 19000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver highest expression Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.76
-
 pH 7.1, temperature not specified in the publication. Coupled enzyme assay, the aldehyde that is produced by hydrolysis of lysoplasmalogen is reduced to an alcohol in a second enzymic reaction. In the second reaction, NADH is oxidized in a 1:1 stoichiometry between the mol of aldehyde produced and mol of NADH oxidized Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lysoplasmenylcholine + H2O
-
 Rattus norvegicus an aldehyde + sn-glycero-3-phosphocholine
-
 ?
lysoplasmenylethanolamine + H2O
-
 Rattus norvegicus aldehyde + sn-glycero-3-phosphoethanolamine
-
 ?
additional information no substrate: 1-alkenyl-2-acyl-sn-glycero-3-phosphocholine or 1-alkenyl-2-acyl-sn-glycero-3-phosphoethanolamine, 1-acyl-sn-glycero-3-phosphocholine or 1-acyl-sn-glycero-3-phosphoethanolamine, and lysophosphatidic acid Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 19000, SDS-PAGE Rattus norvegicus
More enzyme is identical to protein Tmem86b, a hydrophobic transmembrane protein of the YhhN family Rattus norvegicus

Synonyms

Synonyms Comment Organism
Tmem86b
-
 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.3
-
 lysoplasmenylcholine pH 7.1, temperature not specified in the publication. Coupled enzyme assay, the aldehyde that is produced by hydrolysis of lysoplasmalogen is reduced to an alcohol in a second enzymic reaction. In the second reaction, NADH is oxidized in a 1:1 stoichiometry between the mol of aldehyde produced and mol of NADH oxidized Rattus norvegicus
10.3
-
 lysoplasmenylethanolamine pH 7.1, temperature not specified in the publication. Coupled enzyme assay, the aldehyde that is produced by hydrolysis of lysoplasmalogen is reduced to an alcohol in a second enzymic reaction. In the second reaction, NADH is oxidized in a 1:1 stoichiometry between the mol of aldehyde produced and mol of NADH oxidized Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 at least 50% of maximum activity within Rattus norvegicus

General Information

General Information Comment Organism
physiological function overexpression in HEK 293T cells leads to decreased levels of plasmalogens Rattus norvegicus