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Literature summary for 3.3.2.14 extracted from

  • Fida, T.T.; Palamuru, S.; Pandey, G.; Spain, J.C.
    Aerobic biodegradation of 2,4-dinitroanisole by Nocardioides sp. strain JS1661 (2014), Appl. Environ. Microbiol., 80, 7725-7731.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
x * 36000 + y * 34000, SDS-PAGE Nocardioides sp.
36000
-
x * 36000 + y * 34000, SDS-PAGE Nocardioides sp.
234000
-
gel filtration Nocardioides sp.
265000
-
native PAGE Nocardioides sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4-dinitroanisole + H2O Nocardioides sp. the enzyme is involved in the degradation of 2,4-dinitroanisole methanol + 2,4-dinitrophenol
-
?

Organism

Organism UniProt Comment Textmining
Nocardioides sp. A0A096ZEC9 and A0A096ZED0 A0A096ZEC9: 2,4-dinitroanisole hydrolase A, A0A096ZED0: 2,4-dinitroanisole hydrolase B
-

Purification (Commentary)

Purification (Comment) Organism
-
Nocardioides sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:2,4-dinitroanisole-grown cell
-
Nocardioides sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4
-
pH 7.0, temperature not specified in the publication Nocardioides sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitroanisole + H2O the enzyme is involved in the degradation of 2,4-dinitroanisole Nocardioides sp. methanol + 2,4-dinitrophenol
-
?
2,4-dinitroanisole + H2O 2,4-dinitroanisole i.e. 1-methoxy-2,4-dinitrobenzene Nocardioides sp. methanol + 2,4-dinitrophenol
-
?

Subunits

Subunits Comment Organism
multimer x * 36000 + y * 34000, SDS-PAGE Nocardioides sp.

Synonyms

Synonyms Comment Organism
2,4-dinitroanisole ether hydrolase
-
Nocardioides sp.
DNAN demethylase
-
Nocardioides sp.
dnhA
-
Nocardioides sp.
dnhB
-
Nocardioides sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Nocardioides sp.

General Information

General Information Comment Organism
physiological function the constitutive enzyme is involved in the degradation of 2,4-dinitroanisole Nocardioides sp.