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Literature summary for 3.3.2.11 extracted from

  • Sevanian, A.; McLeod, L.L.
    Catalytic properties and inhibition of hepatic cholesterol-epoxide hydrolase (1986), J. Biol. Chem., 261, 54-59.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5,6alpha-epoxy-5alpha-cholestan-3beta-ol competitive to 5,6beta-epoxy-5alpha-cholestan-3beta-ol Rattus norvegicus
5,6beta-epoxy-5beta-cholestan-3beta-ol competitive to 5,6alpha-epoxy-5alpha-cholestan-3beta-ol Rattus norvegicus
6-oxocholestanol
-
Rattus norvegicus
7-oxocholestanol
-
Rattus norvegicus
7-oxocholesterol
-
Rattus norvegicus
cholestane-3beta,5alpha,6beta-triol product inhibition Rattus norvegicus
additional information no inhibition by isoquinoline, styrene oxide, cyclohexane oxide, benzil, cholesterol, and cholic acid Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Rattus norvegicus
0.0037
-
5,6alpha-epoxy-5alpha-cholestan-3beta-ol pH 7.4, 37°C, versus 5,6beta-epoxy-5alpha-cholestan-3beta-ol Rattus norvegicus
0.0044
-
5,6beta-epoxy-5beta-cholestan-3beta-ol pH 7.4, 37°C, versus 5,6alpha-epoxy-5alpha-cholestan-3beta-ol Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O Rattus norvegicus
-
cholestane-3beta,5alpha,6beta-triol
-
?
5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O Rattus norvegicus
-
cholestane-3beta,5alpha,6beta-triol
-
?
additional information Rattus norvegicus the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9 ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
male sprague-dawley rats
-

Purification (Commentary)

Purification (Comment) Organism
partially by microsome preparation Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00045
-
liver microsomes, substrate 5,6beta-epoxy-5alpha-cholestan-3beta-ol Rattus norvegicus
0.00048
-
liver microsomes, substrate 5,6alpha-epoxy-5alpha-cholestan-3beta-ol Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O
-
Rattus norvegicus cholestane-3beta,5alpha,6beta-triol
-
?
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O the enzyme is equally active with both stereoisomers Rattus norvegicus cholestane-3beta,5alpha,6beta-triol
-
?
5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O
-
Rattus norvegicus cholestane-3beta,5alpha,6beta-triol
-
?
5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O the enzyme is equally active with both stereoisomers Rattus norvegicus cholestane-3beta,5alpha,6beta-triol
-
?
additional information the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9 Rattus norvegicus ?
-
?

Synonyms

Synonyms Comment Organism
cholesterol-epoxide hydrolase
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0037
-
5,6beta-epoxy-5beta-cholestan-3beta-ol pH 7.4, 37°C, versus 5,6alpha-epoxy-5alpha-cholestan-3beta-ol Rattus norvegicus
0.0044
-
5,6alpha-epoxy-5alpha-cholestan-3beta-ol pH 7.4, 37°C, versus 5,6beta-epoxy-5alpha-cholestan-3beta-ol Rattus norvegicus